Construct: ORF ccsbBroad304_10616
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005261.1_s304c1, BRDN0000460224
- Derived from:
- ccsbBroadEn_10616
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H1FX-AS1 (339942)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10616
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 339942 | H1FX-AS1 | H1FX antisense RNA 1 | NR_026991.1 | 13.1% | 1_1373del;1665_2211del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 360
- ORF length:
- 291
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggatgggag caggaaaccc aaaagagccg gccctggaac caggtggagg 121 ggaggcagcc cggccatgac ccagagcagg acacctgctc cacttcacct tttgccatga 181 gtaaaagctc cctgaggcct cccaagaagt tgatgccctg tgcttcctgt acagctgcgg 241 aaccggatgg ctttccgtgg ctttgctaca gccactcctg gaagtgctgc ctgactgaat 301 cctcagggca ccccggcagg atggacgtgg tgtaccccct actttacaga tggggaaact 361 tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 421 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 481 ggatta