Construct: ORF ccsbBroad304_10706
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012837.1_s304c1, BRDN0000402723
- Derived from:
- ccsbBroadEn_10706
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CA12 (771)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10706
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 771 | CA12 | carbonic anhydrase 12 | NR_135511.2 | 6.9% | 1_3036del;3210G>C;3460_6112del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 489
- ORF length:
- 423
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac gaagcaacat gaattagggg gcctcttggc cttggtccaa aattgtcaat 121 cagaaatgaa cataaaggac tccagagcag tgggactgtc tgtcaaaaga ctctgtatat 181 cttttgtgga tgagttttgt gagagaacag agagaccatt gtacctggca caagggctct 241 tcatgaaaag ggagacttac tgggaggtgc aagacagtgg catttctcct ctcctcttgc 301 tgctcagcac agccctggat tgcagccccg aggctgagac cagacaaagc ccgggaggca 361 gaaagatgct ccaagaacca acactatcaa tgtctttgca aatcctcaca ggattcctgt 421 gggtccagct ttggaactgg gaaacctttc ttcggatccg cactcattcc actgatgcca 481 gctgcccctg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 541 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 601 tcaacctctg gatta