Construct: ORF ccsbBroad304_10774
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009412.1_s304c1, BRDN0000460233
- Derived from:
- ccsbBroadEn_10774
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DEFA4 (1669)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10774
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1669 | DEFA4 | defensin alpha 4 | NM_001925.3 | 99.6% | 100% | 255T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 360
- ORF length:
- 291
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggattatc gccctcctcg ctgctattct cttggtagcc ctccaggtcc 121 gggcaggccc actccaggca agaggtgatg aggctccagg ccaggagcag cgtgggccag 181 aagaccagga catatctatt tcctttgcat gggataaaag ctctgctctt caggtttcag 241 gctcaacaag gggcatggtc tgctcttgca gattagtatt ctgccggcga acagaacttc 301 gtgttgggaa ctgcctcatt ggcggtgtga gtttcacata ctgctgcacg cgtgtcgatt 361 tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 421 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 481 ggatta