Construct: ORF ccsbBroad304_10995
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006313.1_s304c1, BRDN0000402559
- Derived from:
- ccsbBroadEn_10995
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NF2 (4771)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10995
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4771 | NF2 | neurofibromin 2 | NR_156186.2 | 4% | 1_5420del;5667_6066del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 312
- ORF length:
- 246
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt ggtttcatta cgagcccttc tgctggctct caggcagaag ccccacagca 121 ccgggaccat tcatgaggtc actgcccagc tcatgatgtc cgtgaggctg tccttttggc 181 cagtagccgt gtgcagctgt gtggcacaga tggcttcgtt catcctgatc aaggccccac 241 ctcagccaca gcagtccccc caacctgtgt tgtccaccct attattcatg tacctgccag 301 gccctgctag atacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 361 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 421 caatcaacct ctggatta