Construct: ORF ccsbBroad304_11119
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016421.1_s304c1, BRDN0000460177
- Derived from:
- ccsbBroadEn_11119
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCL3 (6348)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11119
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6348 | CCL3 | C-C motif chemokine ligand 3 | NM_002983.3 | 99.2% | 100% | 6G>A;180C>T |
| 2 | human | 414062 | CCL3L3 | C-C motif chemokine ligand ... | NM_001001437.4 | 94.9% | 94.6% | (many diffs) |
| 3 | human | 6349 | CCL3L1 | C-C motif chemokine ligand ... | NM_021006.5 | 94.9% | 94.6% | (many diffs) |
| 4 | human | 6349 | CCL3L1 | C-C motif chemokine ligand ... | NR_111964.1 | 26.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 345
- ORF length:
- 276
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcaagtctcc actgctgccc ttgctgtcct cctctgcacc atggctctct 121 gcaaccagtt ctctgcatca cttgctgctg acacgccgac cgcctgctgc ttcagctaca 181 cctcccggca gattccacag aatttcatag ctgactactt tgagacgagc agccagtgct 241 ccaagcctgg tgtcatcttc ctaaccaagc gaagccggca ggtctgtgct gaccccagtg 301 aggagtgggt ccagaaatat gtcagcgacc tggagctgag tgccttgcca actttcttgt 361 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 421 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a