Construct: ORF ccsbBroad304_11143
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007416.1_s304c1, BRDN0000408075
- Derived from:
- ccsbBroadEn_11143
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SLC22A4 (6583)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11143
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 105376184 | LOC105376184 | uncharacterized LOC105376184 | XR_001746862.1 | 8.4% | (many diffs) | |
| 2 | human | 105376184 | LOC105376184 | uncharacterized LOC105376184 | XR_930184.2 | 7.1% | (many diffs) | |
| 3 | mouse | 108168691 | LOC108168691 | putative F-box protein At1g... | XM_017318933.1 | 76.7% | 3.2% | (many diffs) |
| 4 | mouse | 434768 | Rhox8 | reproductive homeobox 8 | XM_011251014.1 | 16.3% | 1.3% | (many diffs) |
| 5 | mouse | 434768 | Rhox8 | reproductive homeobox 8 | NM_001004193.2 | 15.1% | 1.2% | (many diffs) |
| 6 | mouse | 71362 | 5430440P10Rik | RIKEN cDNA 5430440P10 gene | NR_045859.1 | 13.9% | (many diffs) | |
| 7 | mouse | 102637118 | Gm34011 | predicted gene, 34011 | XR_386243.1 | 8.1% | (many diffs) | |
| 8 | mouse | 102639254 | Gm35607 | predicted gene, 35607 | XR_877472.2 | 5.3% | (many diffs) | |
| 9 | mouse | 102636562 | Gm33594 | predicted gene, 33594 | XR_374379.3 | 4.5% | (many diffs) | |
| 10 | mouse | 108168202 | Gm46486 | predicted gene, 46486 | XR_001781474.1 | 2.9% | (many diffs) | |
| 11 | mouse | 105245994 | Gm41361 | predicted gene, 41361 | XR_001781721.1 | 2.1% | (many diffs) | |
| 12 | mouse | 102636562 | Gm33594 | predicted gene, 33594 | XR_374378.3 | 1.9% | (many diffs) | |
| 13 | mouse | 108168402 | Gm46642 | predicted gene, 46642 | XR_001782550.1 | 1.9% | (many diffs) | |
| 14 | mouse | 102635541 | Gm32849 | predicted gene, 32849 | XR_391458.3 | .1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 231
- ORF length:
- 165
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggaatgcg cctcgcaagc caaagaagaa gaagaggaag aggaaagaag aagaagagga 121 agaggaagaa gaagaagaag aagaagaaga agaagaagaa gaagaagaag aagaagaaga 181 agaagaaaag aagaagaaga agaagaggaa gaagaagaag aatgcagcag taggcaactt 241 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 301 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta