Construct: ORF ccsbBroad304_11261
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009345.1_s304c1, BRDN0000405561
- Derived from:
- ccsbBroadEn_11261
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GTPBP6 (8225)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11261
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8225 | GTPBP6 | GTP binding protein 6 (puta... | XM_006724447.4 | 93.4% | 93.3% | 0_1ins78;94A>G |
| 2 | human | 8225 | GTPBP6 | GTP binding protein 6 (puta... | XM_006724868.4 | 93.4% | 93.3% | 0_1ins78;94A>G |
| 3 | human | 8225 | GTPBP6 | GTP binding protein 6 (puta... | XM_011545637.3 | 83.3% | 83.3% | 0_1ins201 |
| 4 | human | 8225 | GTPBP6 | GTP binding protein 6 (puta... | XM_011546184.3 | 83.3% | 83.3% | 0_1ins201 |
| 5 | human | 8225 | GTPBP6 | GTP binding protein 6 (puta... | NM_012227.3 | 78% | 77.9% | 1_339del;511A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1275
- ORF length:
- 1209
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac tcgagccgag tggcaggtgg cggaggccac agcgctggtg cacacgctgg 121 acggctggtc cgtggtgcag acaatggtcg tgtccaccaa aacgccggac aggaagctca 181 tctttggcaa agggaacttt gagcacctga cagaaaagat ccgagggtct ccagacgtca 241 cgtgcgtctt cctgaacgtg gagaggatgg ctgccccgac caagaaagaa ctggaagccg 301 cctggggcgt ggaggtgttt gaccgcttca cggtcgtcct gcacatcttc cgctgtaacg 361 cccgcacgaa ggaggcccgg cttcaggtgg ccctggcgga gatgccgctg cacaggtcga 421 acttgaaaag ggacgtcgcc cacctgtacc gaggagtcgg ctcgcgctac atcatggggt 481 caggagaatc cttcatgcag ctgcagcagc gtctcctgag agagaaggag gccaagatca 541 ggaaggcctt ggacaggctt cgcaagaaga ggcacctgct ccgccggcag cggacgaggc 601 gggagttccc cgtgatctcc gtggtggggt acaccaactg cggaaagacc acgctgatca 661 aggcactgac gggcgatgcc gccatccagc cacgggacca gctgtttgcc acgctggacg 721 tcacggccca cgcgggcacg ctgccctcac gcatgaccgt cctgtacgtg gacaccatcg 781 gcttcctctc ccagctgccg cacggcctca tcgagtcctt ctccgccacc ctggaagacg 841 tggcccactc ggatctcatc ttgcacgtga gggacgtcag ccaccccgag gcggagctcc 901 agaaatgcag cgttctgtcc acgctgcgtg gcctgcagct gcccgccccg ctcctggact 961 ccatggtgga ggttcacaac aaggtggacc tcgtgcccgg gtacagcccc acggaaccga 1021 acgtcgtgcc cgtgtctgcc ctgcggggcc acgggctcca ggagctgaaa gctgagctcg 1081 atgcggcggt tttgaaggcg acggggagac agatcctcac tctccgtgtg aggctcgcag 1141 gggcgcagct cagctggctg tataaggagg ccacagttca ggaggtggac gtgatccctg 1201 aggacggggc ggccgacgtg agggtcatca tcagcaactc agcctacggc aaattccgga 1261 agctctttcc aggatgaaca actttcttgt acaaagtggt tggtaagcct atccctaacc 1321 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 1381 cgacaatcaa cctctggatt a