Construct: ORF ccsbBroad304_11295
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015758.1_s304c1, BRDN0000400537
- Derived from:
- ccsbBroadEn_11295
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDC23 (8697)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11295
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8697 | CDC23 | cell division cycle 23 | NM_004661.4 | 98.9% | 98.9% | 1_18del |
| 2 | mouse | 52563 | Cdc23 | CDC23 cell division cycle 23 | NM_178347.4 | 87.6% | 94.8% | (many diffs) |
| 3 | mouse | 52563 | Cdc23 | CDC23 cell division cycle 23 | XM_006526084.3 | 83.7% | 90.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1839
- ORF length:
- 1773
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt cccggtggct gtgacggcgg cagtggcgcc tgtcctgtcc ataaacagcg 121 atttctcaga tttgcgggaa attaaaaagc aactgctgct tattgcgggc cttacccggg 181 agcggggcct actacacagt agcaaatggt cggcggagtt ggctttctct ctccctgcat 241 tgcctctggc cgagctgcaa ccgcctccgc ctattacaga ggaagatgcc caggatatgg 301 atgcctatac cctggccaag gcctactttg acgttaaaga gtatgatcgg gcagcacatt 361 tcctgcatgg ctgcaatagc aagaaagcct attttctgta tatgtattcc agatatctgt 421 ctggagaaaa aaagaaggac gatgaaacag ttgatagctt aggccccctg gaaaaaggac 481 aagtgaaaaa tgaggcgctt agagaattga gagtggagct cagcaaaaaa caccaagctc 541 gagaacttga tggatttgga ctttatctgt atggtgtggt gcttcgaaaa ctggacttgg 601 ttaaagaggc cattgatgtg tttgtggaag ctactcatgt tttgcccttg cattggggag 661 cctggttaga actctgtaac ctgatcacag acaaagagat gctgaagttc ctgtctttgc 721 cagacacctg gatgaaagag ttttttctgg ctcatatata cacagagttg cagttgatag 781 aggaggccct gcaaaagtat cagaatctca ttgatgtggg cttctctaag agctcgtata 841 ttgtttccca aattgcagtt gcctatcaca atatcagaga tattgacaaa gccctctcca 901 tttttaatga gctaaggaaa caagaccctt acaggattga aaatatggac acattctcca 961 accttcttta tgtcaggagc atgaaatcgg agttgagtta tctggctcat aacctctgtg 1021 agattgataa atatcgtgta gaaacgtgct gtgtaattgg caattattac agtttacgtt 1081 ctcagcatga gaaagcagcc ttatatttcc agagagccct gaaattaaat cctcggtatc 1141 ttggtgcctg gacactaatg ggacatgagt acatggagat gaagaacacg tctgctgcta 1201 tccaggctta tagacatgcc attgaggtca acaaacggga ctacagagct tggtatggcc 1261 tcgggcagac ctatgaaatc cttaagatgc cattttactg cctttattat tatagacggg 1321 cccaccagct tcgacccaat gattctcgca tgctggttgc tttaggagaa tgttacgaga 1381 aactcaatca actagtggaa gccaaaaagt gttattggag agcttacgcc gtgggagatg 1441 tggagaaaat ggctctggtg aaactggcaa agcttcatga acagttgact gagtcagaac 1501 aggctgccca gtgttacatc aaatatatcc aagatatcta ttcctgtggg gaaatagtag 1561 aacacttgga ggaaagcact gcctttcgct atctggccca gtactatttt aagtgcaaac 1621 tgtgggatga agcttcaact tgtgcacaaa agtgttgtgc atttaatgat acccgggaag 1681 aaggtaaggc cttactccgg caaatcctac agcttcggaa ccaaggcgag actcctacca 1741 ccgaggtgcc tgctcccttt ttcctacctg cttcactctc tgctaacaat acccccacac 1801 gcagagtttc tccactcaac ttgtcttctg tcacgccata cccaactttc ttgtacaaag 1861 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1921 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta