Construct: ORF ccsbBroad304_11313
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005594.1_s304c1, BRDN0000402668
- Derived from:
- ccsbBroadEn_11313
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APLN (8862)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11313
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8862 | APLN | apelin | NM_017413.5 | 24.8% | 11.7% | 1_112del;231_232ins247 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 432
- ORF length:
- 366
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc cgccacctgg tgcagcccag agggtcaagg aatgggccag ggccctggca 121 gggaggtcgg aggaaattcc gccgccagcg gccccgcctc tcccataagg gacccatgcc 181 tttctgaagc aggactgaag gggcccccaa gtgcccaccc ccggcggtta tgtctcctcc 241 atagattggt ctgcttctct ggaggcctca cgtccattca gctctcacct cgcacctgct 301 gtagccacca gtgggcccag ctcttctcac ctgcctgctt cccccagtgg cgtgctcctg 361 gctgtagttt ggatgattcc cgttctctca caagaatccg tccagtccat cttcctggcc 421 cctccctgga ctgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 481 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 541 caatcaacct ctggatta