Construct: ORF ccsbBroad304_11386
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006138.1_s304c1, BRDN0000404525
- Derived from:
- ccsbBroadEn_11386
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CIR1 (9541)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11386
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9541 | CIR1 | corepressor interacting wit... | NM_004882.4 | 41.9% | 38.6% | 483_484ins28;579_1350del |
| 2 | mouse | 66935 | Cir1 | corepressor interacting wit... | NM_025854.3 | 38.6% | 39.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 672
- ORF length:
- 606
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg gaaatccttc gccaacttca tgtgcaagaa agactttcat cctgcctcca 121 aatccaatat caaaaaagta tggatggcag aacagaaaat atcatatgat aagaagaaac 181 aagaagaatt gatgcagcaa tatcttaaag aacaagaatc atatgataat agattgctta 241 tgggagatga acgtgtaaag aatggcctta atttcatgta tgaagcccca ccaggagcta 301 aaaaagaaaa caaagagaaa gaagaaacag aaggagagac cgaatacaaa tttgaatggc 361 agaaaggagc cccacgagaa aaatatgcca aagatgacat gaacatcaga gatcagccct 421 ttggtattca ggttcgaaat gtgaggtgca ttaaatgtca caaatggggt catgtcaaca 481 cagatcgaga atgtcctttg tttggtcttt ctggaatcaa tgcaagttcg gttcccactg 541 atggctcaga tgctgtgtta caaattgaag gtttctgggc catcgatgca cccctcggag 601 ctaatagcgg agatgagaaa cagtgggttt gcactgaaac gaaatgtact ggggagaaac 661 ttgaccgcaa atgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 721 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 781 caatcaacct ctggatta