Construct: ORF ccsbBroad304_11436
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005361.1_s304c1, BRDN0000398752
- Derived from:
- ccsbBroadEn_11436
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRYZL1 (9946)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11436
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9946 | CRYZL1 | crystallin zeta like 1 | NM_145858.3 | 29% | 26% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 405
- ORF length:
- 339
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa aggcttatat ttccaacaga gttccacaga tgaagaaata acatttgtat 121 ttcaagaaaa ggaagatctt cctgttacag aggataactt tgtgaaactt caagttaaag 181 cttgtgctct gagccagata aatacaaagc ttctggcaga aatgaagatg aaaaaggatt 241 tatttcctgt tgggagagaa attgctggaa ttgtattaga tgttggaagc aaggtatcat 301 tctttcaacc agatgatgaa gtagttgaag tactttcccg gctgggtgct gtggctcacg 361 tctgtaatcc cagcacgttg ggaggccaag gtgggcgaat cacctgcccc actttcttgt 421 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 481 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a