Construct: ORF ccsbBroad304_11492
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001297.1_s304c1, BRDN0000405914
- Derived from:
- ccsbBroadEn_11492
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDRG1 (10397)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11492
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10397 | NDRG1 | N-myc downstream regulated 1 | XM_011516792.2 | 53.1% | 53.1% | 1_288del |
| 2 | human | 10397 | NDRG1 | N-myc downstream regulated 1 | NM_001258433.1 | 34.8% | 34.8% | 1_612del |
| 3 | human | 10397 | NDRG1 | N-myc downstream regulated 1 | NM_001258432.2 | 33.2% | 33.2% | 1_657del |
| 4 | human | 10397 | NDRG1 | N-myc downstream regulated 1 | NM_001135242.2 | 27.6% | 27.6% | 1_855del |
| 5 | human | 10397 | NDRG1 | N-myc downstream regulated 1 | NM_006096.4 | 27.6% | 27.6% | 1_855del |
| 6 | human | 10397 | NDRG1 | N-myc downstream regulated 1 | XM_011516791.1 | 26.5% | 26.5% | 1_906del |
| 7 | mouse | 17988 | Ndrg1 | N-myc downstream regulated ... | NM_008681.2 | 24.1% | 25.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 393
- ORF length:
- 327
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggactgtggc ggcctcccgc agatctccca gccggccaag ctcgctgagg 121 ccttcaagta cttcgtgcag ggcatgggat acatgccctc ggctagcatg acccgcctga 181 tgcggtcccg cacagcctct ggttccagcg tcacttctct ggatggcacc cgcagccgct 241 cccacaccag cgagggcacc cgaagccgct cccacaccag cgagggcacc cgcagccgct 301 cgcacaccag cgagggggcc cacctggaca tcacccccaa ctcgggtgct gctgggaaca 361 gcgccgggcc caagtccatg gaggtctcct gctacccaac tttcttgtac aaagtggttg 421 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 481 accggtggcg cgttaagtcg acaatcaacc tctggatta