Construct: ORF ccsbBroad304_11534
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016757.1_s304c1, BRDN0000408058
- Derived from:
- ccsbBroadEn_11534
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IGF2BP3 (10643)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11534
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10643 | IGF2BP3 | insulin like growth factor ... | NM_006547.3 | 16.8% | 16.4% | (many diffs) |
| 2 | human | 10643 | IGF2BP3 | insulin like growth factor ... | XM_011515089.2 | 16.8% | 16.3% | (many diffs) |
| 3 | mouse | 140488 | Igf2bp3 | insulin-like growth factor ... | NM_023670.3 | 15.3% | 14.8% | (many diffs) |
| 4 | mouse | 140488 | Igf2bp3 | insulin-like growth factor ... | XR_377421.3 | 12.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 366
- ORF length:
- 300
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa caaactgtat atcggaaacc tcagcgagaa cgccgccccc tcggacctag 121 aaagtatctt caaggacgcc aagatcccgg tgtcgggacc cttcctggtg aagactggct 181 acgcgttcgt ggactgcccg gacgagagct gggccctcaa ggccatcgag gcgctttcag 241 gtaaaataga actgcacggg aaacccatag aagttgagca ctcggtccca aaaaggcaaa 301 ggattcggaa acttcagata cgaaatatcc cgcctcattt acagtgggag gaaatggggc 361 agccttgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 421 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 481 acctctggat ta