Construct: ORF ccsbBroad304_11646
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001453.1_s304c1, BRDN0000400132
- Derived from:
- ccsbBroadEn_11646
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DHX30 (22907)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11646
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 22907 | DHX30 | DExH-box helicase 30 | NM_014966.3 | 43.3% | 43.2% | 1_1962del;3289C>T |
2 | human | 22907 | DHX30 | DExH-box helicase 30 | XM_017005917.1 | 43.3% | 43.2% | 1_1962del;3289C>T |
3 | human | 22907 | DHX30 | DExH-box helicase 30 | XM_017005916.2 | 43.2% | 43.2% | 1_1968del;3295C>T |
4 | human | 22907 | DHX30 | DExH-box helicase 30 | XM_006713033.1 | 43% | 43% | 1_1983del;3310C>T |
5 | human | 22907 | DHX30 | DExH-box helicase 30 | XM_017005915.1 | 43% | 43% | 1_1983del;3310C>T |
6 | human | 22907 | DHX30 | DExH-box helicase 30 | NM_001330990.1 | 42.9% | 42.8% | 1_1995del;3322C>T |
7 | human | 22907 | DHX30 | DExH-box helicase 30 | XM_011533497.2 | 42.9% | 42.8% | 1_1995del;3322C>T |
8 | human | 22907 | DHX30 | DExH-box helicase 30 | XM_011533498.1 | 42.9% | 42.8% | 1_1995del;3322C>T |
9 | human | 22907 | DHX30 | DExH-box helicase 30 | NM_138615.3 | 41.9% | 41.8% | 1_2079del;3406C>T |
10 | human | 22907 | DHX30 | DExH-box helicase 30 | XM_011533494.3 | 41.9% | 41.8% | 1_2079del;3406C>T |
11 | human | 22907 | DHX30 | DExH-box helicase 30 | XM_011533495.2 | 41.9% | 41.8% | 1_2079del;3406C>T |
12 | human | 22907 | DHX30 | DExH-box helicase 30 | XM_024453405.1 | 40.7% | 40.7% | 1_2181del;3508C>T |
13 | human | 22907 | DHX30 | DExH-box helicase 30 | XM_017005914.1 | 40.4% | 40.3% | 1_2211del;3538C>T |
14 | human | 22907 | DHX30 | DExH-box helicase 30 | XM_011533490.2 | 39.5% | 39.5% | 1_2292del;3619C>T |
15 | mouse | 72831 | Dhx30 | DEAH (Asp-Glu-Ala-His) box ... | XM_006512310.3 | 37.4% | 41.3% | (many diffs) |
16 | mouse | 72831 | Dhx30 | DEAH (Asp-Glu-Ala-His) box ... | XM_017313615.1 | 37.4% | 41.3% | (many diffs) |
17 | mouse | 72831 | Dhx30 | DEAH (Asp-Glu-Ala-His) box ... | XM_017313616.1 | 37.4% | 41.3% | (many diffs) |
18 | mouse | 72831 | Dhx30 | DEAH (Asp-Glu-Ala-His) box ... | NM_001252682.1 | 37.2% | 41.1% | (many diffs) |
19 | mouse | 72831 | Dhx30 | DEAH (Asp-Glu-Ala-His) box ... | XM_006512303.1 | 37.2% | 41.1% | (many diffs) |
20 | mouse | 72831 | Dhx30 | DEAH (Asp-Glu-Ala-His) box ... | XM_006512304.1 | 37.2% | 41.1% | (many diffs) |
21 | mouse | 72831 | Dhx30 | DEAH (Asp-Glu-Ala-His) box ... | XM_006512305.3 | 37.2% | 41.1% | (many diffs) |
22 | mouse | 72831 | Dhx30 | DEAH (Asp-Glu-Ala-His) box ... | NM_133347.2 | 36.5% | 40.3% | (many diffs) |
23 | mouse | 72831 | Dhx30 | DEAH (Asp-Glu-Ala-His) box ... | XM_006512298.3 | 36.5% | 40.3% | (many diffs) |
24 | mouse | 72831 | Dhx30 | DEAH (Asp-Glu-Ala-His) box ... | XM_006512299.1 | 36.5% | 40.3% | (many diffs) |
25 | mouse | 72831 | Dhx30 | DEAH (Asp-Glu-Ala-His) box ... | XM_006512300.1 | 36.5% | 40.3% | (many diffs) |
26 | mouse | 72831 | Dhx30 | DEAH (Asp-Glu-Ala-His) box ... | XM_006512301.3 | 36.5% | 40.3% | (many diffs) |
27 | mouse | 72831 | Dhx30 | DEAH (Asp-Glu-Ala-His) box ... | XM_011243029.2 | 36.5% | 40.3% | (many diffs) |
28 | mouse | 72831 | Dhx30 | DEAH (Asp-Glu-Ala-His) box ... | XM_017313613.1 | 36.5% | 40.3% | (many diffs) |
29 | mouse | 72831 | Dhx30 | DEAH (Asp-Glu-Ala-His) box ... | XM_017313614.1 | 36.5% | 40.3% | (many diffs) |
30 | mouse | 72831 | Dhx30 | DEAH (Asp-Glu-Ala-His) box ... | NM_001252683.1 | 36.3% | 40.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1569
- ORF length:
- 1503
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca cgagagcaag tacctcatcc tgccagtgca ctccaacatc cccatgatgg 121 atcagaaggc catattccag cagcctccag ttggggtgcg caagattgtc ttggccacca 181 acattgctga gacttccatc acaatcaatg acatcgtgca tgtggtggac agtgggctgc 241 acaaggaaga acgctatgac ctgaagacca aggtgtcctg cctggagaca gtgtgggtat 301 caagagccaa tgtgatccag cgccggggcc gggcgggccg ctgccagtcc ggctttgcct 361 accacttgtt ccctcgaagc cggctggaga aaatggtccc tttccaagtg ccagagatcc 421 tgcgcacacc tcttgagaac ctggtgctgc aagcgaaaat ccacatgcct gagaagacgg 481 cggtggagtt cctgtccaag gctgtggaca gtccaaacat caaggcagtg gacgaggctg 541 tgatcttgct ccaggagatc ggggtgctgg accagcggga gtacctgact accctggggc 601 agcgcctggc tcacatctcc accgaccccc ggttggccaa ggccattgtg ttggctgcca 661 tcttccgttg cctgcaccca ctactggtgg tcgtttcctg cctcacccgg gaccccttca 721 gcagcagcct acagaaccgg gcagaggtgg acaaggtgaa agcactgttg agccatgaca 781 gcggcagtga ccacctggcc tttgtgcggg ctgtcgccgg ctgggaggag gtgctgcgtt 841 ggcaggaccg cagctcccgg gagaattacc tggaggaaaa cctgctgtac gcacccagcc 901 tgcgcttcat ccacggactc atcaagcagt tctcagagaa catttatgag gccttcctgg 961 tggggaagcc ctcggactgc accctggcct ccgcccagtg caacgagtac agtgaggagg 1021 aggagctggt gaagggcgtg ctgatggccg gcctctaccc caacctcatc caggtgaggc 1081 agggcaaggt cacccggcag gggaagttca agcccaacag cgtcacatat aggaccaaat 1141 caggcaacat cctgctgcac aagtcgacca ttaacaggga ggccacacgg ttacggagcc 1201 gatggctgac gtatttcatg gcagtcaagt ccaatggcag cgtcttcgtc cgggactcct 1261 ctcaggtgca cccgctagct gtgctgctgc tgaccgacgg ggacgtgcac atccgtgatg 1321 acgggcgccg ggccaccatc tcactgagcg acagtgacct gctgcggctg gagggtgact 1381 cgcgtaccgt gtggctgctg aaggagctgc ggcgggccct gggccgcatg gtggagcgga 1441 gcctgcgcag cgagctggct gcacttcccc ccagcgtaca ggaggagcac gggcagctgc 1501 ttgcgctact ggcagagctg ctgcgaggac cctgtggcag ctttgatgtg cgcaagacag 1561 ctgacgactg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1621 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1681 tcaacctctg gatta