Construct: ORF ccsbBroad304_11646

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001453.1_s304c1, BRDN0000400132
Derived from:
ccsbBroadEn_11646
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DHX30 (22907)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11646
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 22907 DHX30 DExH-box helicase 30 NM_014966.3 43.3% 43.2% 1_1962del;3289C>T
2 human 22907 DHX30 DExH-box helicase 30 XM_017005917.1 43.3% 43.2% 1_1962del;3289C>T
3 human 22907 DHX30 DExH-box helicase 30 XM_017005916.2 43.2% 43.2% 1_1968del;3295C>T
4 human 22907 DHX30 DExH-box helicase 30 XM_006713033.1 43% 43% 1_1983del;3310C>T
5 human 22907 DHX30 DExH-box helicase 30 XM_017005915.1 43% 43% 1_1983del;3310C>T
6 human 22907 DHX30 DExH-box helicase 30 NM_001330990.1 42.9% 42.8% 1_1995del;3322C>T
7 human 22907 DHX30 DExH-box helicase 30 XM_011533497.2 42.9% 42.8% 1_1995del;3322C>T
8 human 22907 DHX30 DExH-box helicase 30 XM_011533498.1 42.9% 42.8% 1_1995del;3322C>T
9 human 22907 DHX30 DExH-box helicase 30 NM_138615.3 41.9% 41.8% 1_2079del;3406C>T
10 human 22907 DHX30 DExH-box helicase 30 XM_011533494.3 41.9% 41.8% 1_2079del;3406C>T
11 human 22907 DHX30 DExH-box helicase 30 XM_011533495.2 41.9% 41.8% 1_2079del;3406C>T
12 human 22907 DHX30 DExH-box helicase 30 XM_024453405.1 40.7% 40.7% 1_2181del;3508C>T
13 human 22907 DHX30 DExH-box helicase 30 XM_017005914.1 40.4% 40.3% 1_2211del;3538C>T
14 human 22907 DHX30 DExH-box helicase 30 XM_011533490.2 39.5% 39.5% 1_2292del;3619C>T
15 mouse 72831 Dhx30 DEAH (Asp-Glu-Ala-His) box ... XM_006512310.3 37.4% 41.3% (many diffs)
16 mouse 72831 Dhx30 DEAH (Asp-Glu-Ala-His) box ... XM_017313615.1 37.4% 41.3% (many diffs)
17 mouse 72831 Dhx30 DEAH (Asp-Glu-Ala-His) box ... XM_017313616.1 37.4% 41.3% (many diffs)
18 mouse 72831 Dhx30 DEAH (Asp-Glu-Ala-His) box ... NM_001252682.1 37.2% 41.1% (many diffs)
19 mouse 72831 Dhx30 DEAH (Asp-Glu-Ala-His) box ... XM_006512303.1 37.2% 41.1% (many diffs)
20 mouse 72831 Dhx30 DEAH (Asp-Glu-Ala-His) box ... XM_006512304.1 37.2% 41.1% (many diffs)
21 mouse 72831 Dhx30 DEAH (Asp-Glu-Ala-His) box ... XM_006512305.3 37.2% 41.1% (many diffs)
22 mouse 72831 Dhx30 DEAH (Asp-Glu-Ala-His) box ... NM_133347.2 36.5% 40.3% (many diffs)
23 mouse 72831 Dhx30 DEAH (Asp-Glu-Ala-His) box ... XM_006512298.3 36.5% 40.3% (many diffs)
24 mouse 72831 Dhx30 DEAH (Asp-Glu-Ala-His) box ... XM_006512299.1 36.5% 40.3% (many diffs)
25 mouse 72831 Dhx30 DEAH (Asp-Glu-Ala-His) box ... XM_006512300.1 36.5% 40.3% (many diffs)
26 mouse 72831 Dhx30 DEAH (Asp-Glu-Ala-His) box ... XM_006512301.3 36.5% 40.3% (many diffs)
27 mouse 72831 Dhx30 DEAH (Asp-Glu-Ala-His) box ... XM_011243029.2 36.5% 40.3% (many diffs)
28 mouse 72831 Dhx30 DEAH (Asp-Glu-Ala-His) box ... XM_017313613.1 36.5% 40.3% (many diffs)
29 mouse 72831 Dhx30 DEAH (Asp-Glu-Ala-His) box ... XM_017313614.1 36.5% 40.3% (many diffs)
30 mouse 72831 Dhx30 DEAH (Asp-Glu-Ala-His) box ... NM_001252683.1 36.3% 40.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1569
ORF length:
1503
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgca cgagagcaag tacctcatcc tgccagtgca ctccaacatc cccatgatgg
121atcagaaggc catattccag cagcctccag ttggggtgcg caagattgtc ttggccacca
181acattgctga gacttccatc acaatcaatg acatcgtgca tgtggtggac agtgggctgc
241acaaggaaga acgctatgac ctgaagacca aggtgtcctg cctggagaca gtgtgggtat
301caagagccaa tgtgatccag cgccggggcc gggcgggccg ctgccagtcc ggctttgcct
361accacttgtt ccctcgaagc cggctggaga aaatggtccc tttccaagtg ccagagatcc
421tgcgcacacc tcttgagaac ctggtgctgc aagcgaaaat ccacatgcct gagaagacgg
481cggtggagtt cctgtccaag gctgtggaca gtccaaacat caaggcagtg gacgaggctg
541tgatcttgct ccaggagatc ggggtgctgg accagcggga gtacctgact accctggggc
601agcgcctggc tcacatctcc accgaccccc ggttggccaa ggccattgtg ttggctgcca
661tcttccgttg cctgcaccca ctactggtgg tcgtttcctg cctcacccgg gaccccttca
721gcagcagcct acagaaccgg gcagaggtgg acaaggtgaa agcactgttg agccatgaca
781gcggcagtga ccacctggcc tttgtgcggg ctgtcgccgg ctgggaggag gtgctgcgtt
841ggcaggaccg cagctcccgg gagaattacc tggaggaaaa cctgctgtac gcacccagcc
901tgcgcttcat ccacggactc atcaagcagt tctcagagaa catttatgag gccttcctgg
961tggggaagcc ctcggactgc accctggcct ccgcccagtg caacgagtac agtgaggagg
1021aggagctggt gaagggcgtg ctgatggccg gcctctaccc caacctcatc caggtgaggc
1081agggcaaggt cacccggcag gggaagttca agcccaacag cgtcacatat aggaccaaat
1141caggcaacat cctgctgcac aagtcgacca ttaacaggga ggccacacgg ttacggagcc
1201gatggctgac gtatttcatg gcagtcaagt ccaatggcag cgtcttcgtc cgggactcct
1261ctcaggtgca cccgctagct gtgctgctgc tgaccgacgg ggacgtgcac atccgtgatg
1321acgggcgccg ggccaccatc tcactgagcg acagtgacct gctgcggctg gagggtgact
1381cgcgtaccgt gtggctgctg aaggagctgc ggcgggccct gggccgcatg gtggagcgga
1441gcctgcgcag cgagctggct gcacttcccc ccagcgtaca ggaggagcac gggcagctgc
1501ttgcgctact ggcagagctg ctgcgaggac cctgtggcag ctttgatgtg cgcaagacag
1561ctgacgactg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc
1621tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
1681tcaacctctg gatta

Download FASTA (ORF) (Full)