Construct: ORF ccsbBroad304_11652
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016964.1_s304c1, BRDN0000401192
- Derived from:
- ccsbBroadEn_11652
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POMZP3 (22932)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11652
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22932 | POMZP3 | POM121 and ZP3 fusion | NM_012230.3 | 88.4% | 87.6% | (many diffs) |
| 2 | human | 22932 | POMZP3 | POM121 and ZP3 fusion | NM_152992.2 | 54.6% | 54.2% | 313G>C;345_346ins285 |
| 3 | human | 7784 | ZP3 | zona pellucida glycoprotein 3 | NM_007155.6 | 49.1% | 38.8% | (many diffs) |
| 4 | human | 7784 | ZP3 | zona pellucida glycoprotein 3 | NM_001110354.2 | 43.2% | 34.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 696
- ORF length:
- 630
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt gtgtagccca gtgactctga ggatcgcccc tcctgacaga agattttcgc 121 gttctgcgat accagagcag ataatcagct caacactgtc ctcaccatca agtaatgccc 181 cagacccatg tgcaaaggag actgtactga gtgccctcaa agagaagaag aagaaaagga 241 cagtggagga agaagaccaa atattccttg atggccagga aaataaaaga agctgtcttg 301 tcgacggtct cactgatgcc tcttctgcat tcaaagttcc tcgacccggg ccagatacac 361 tccagttcac agtggatctc ttccactttg ctaatgactc cagaaacatg atatacatca 421 cctgccacct gaaggtcacc ctagctgagc aggacccaga tgaactcaac aaggcctgtt 481 ccttcagcaa gccttccaac agctggttcc cagtggaagg cccggctgac atctgtcaat 541 gctgtaacaa aggtgactgt ggcactccaa gccattccag gaggcagcct cgtgtcgtga 601 gccagtggtc cacgtctgct tcccgtaacc gcaggcatgt gacagaagaa gcagatgtca 661 ccgtgggggc cactgatctt cctggacagg agtggtgccc aactttcttg tacaaagtgg 721 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 781 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta