Construct: ORF ccsbBroad304_11695
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009058.1_s304c1, BRDN0000398673
- Derived from:
- ccsbBroadEn_11695
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MLC1 (23209)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11695
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23209 | MLC1 | modulator of VRAC current 1 | XR_001755181.2 | 8.4% | 1_1610del;1806_2299del | |
| 2 | human | 23209 | MLC1 | modulator of VRAC current 1 | XR_001755180.2 | 7.7% | 1_1842del;2038_2531del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 261
- ORF length:
- 195
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca tctgtggcct ggggccatca agatcaaaga accaggaggc ctgggagatg 121 cagctggatg gggcggcctg cagaccctgc cagggggttt gaggaccctc ccaggtttcc 181 cactgcggaa caggagtgac tctggctgcc aagatacctt catggtgttc atgacaagtg 241 gaatcattat tttcaaccat tgcccaactt tcttgtacaa agtggttggt aagcctatcc 301 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 361 ttaagtcgac aatcaacctc tggatta