Construct: ORF ccsbBroad304_12066
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010369.1_s304c1, BRDN0000403314
- Derived from:
- ccsbBroadEn_12066
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HES2 (54626)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12066
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54626 | HES2 | hes family bHLH transcripti... | NM_019089.5 | 37.7% | 29.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 294
- ORF length:
- 228
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg gctgcctcgc cgggcagggg acgcggcgga gctgcgcaag agcctgaagc 121 cgctgctgga gaagcgccgg cgcgcgcgca tcaaccagag cctgagccag cttaaggggc 181 tcatcctgcc gctgctgggc cgggaggatg cttctggctg gcacacctgg cttcccctcc 241 atgctcagaa ctgcttccta ctctacatcc aggctcctga gcagccccca gcttacccaa 301 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 361 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta