Construct: ORF ccsbBroad304_12104

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014557.1_s304c1, BRDN0000405655
Derived from:
ccsbBroadEn_12104
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NSUN2 (54888)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12104
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54888 NSUN2 NOP2/Sun RNA methyltransfer... NM_001193455.2 77.3% 77.3% 1_498del
2 human 54888 NSUN2 NOP2/Sun RNA methyltransfer... NM_017755.6 73.7% 73.7% 1_603del
3 human 54888 NSUN2 NOP2/Sun RNA methyltransfer... NR_037947.2 56.9% (many diffs)
4 mouse 28114 Nsun2 NOL1/NOP2/Sun domain family... NM_145354.5 60.9% 63.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1764
ORF length:
1698
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa tgtccccttt ccagagggat ttgttattgc gaatgatgtg gacaacaagc
121gctgctacct gctcgtccat caagccaaga ggctgagcag cccctgcatc atggtggtca
181accatgatgc ctccagcata cccaggctcc agatagatgt ggacggcagg aaagagatcc
241tcttctatga tcgaatttta tgtgatgtcc cttgcagtgg agacggcact atgagaaaaa
301acattgatgt ttggaaaaag tggaccacct taaatagctt gcagctacat ggcttacagc
361tgcggattgc aacacgcggg gctgaacagc tggctgaagg tggaaggatg gtgtattcca
421cgtgttcact aaaccctatt gaggatgaag cagtcatagc atctttactg gaaaaaagtg
481aaggtgcttt ggagcttgct gatgtgtcta atgaactgcc agggctgaag tggatgcctg
541gaatcacaca gtggaaggta atgacgaaag atgggcagtg gtttacagac tgggacgctg
601ttcctcacag cagacacacc cagatccgac ctaccatgtt ccctccgaag gacccagaaa
661agctgcaggc catgcacctg gagcgatgcc ttaggatatt accccatcat cagaatactg
721gagggttttt tgtggcagta ttggtgaaaa aatcttcaat gccgtggaat aaacgtcagc
781caaagcttca gggtaaatct gcagagacca gagaaagcac acagctgagc cctgcagatc
841tcacagaagg gaaacccaca gatccctcta agctggaaag tccgtcattc acaggaactg
901gtgacacaga aatagctcat gcaactgagg atttagagaa taatggcagt aagaaagatg
961gcgtgtgtgg tcctcctcca tcaaagaaaa tgaagttatt tggatttaaa gaagatccat
1021ttgtatttat tcctgaagat gacccattat ttccacctat tgagaaattt tatgctttgg
1081atccttcatt cccaaggatg aatttgttaa ctcggactac agaagggaag aaaaggcagc
1141tctacatggt ttctaaggag ttgcggaatg tgctgctgaa taacagtgag aagatgaagg
1201ttattaacac ggggatcaaa gtctggtgta gaaataacag cggtgaagag tttgactgtg
1261ctttccggct ggcacaggag ggaatatata cattgtatcc atttattaac tcaagaatta
1321ttactgtatc aatggaagat gttaagatac tgttgaccca ggaaaatccc ttttttagaa
1381aactcagcag tgagacctac agtcaagcaa aggacctggc aaagggaagc atcgtgctga
1441agtatgaacc agattctgcg aatccagacg ctctgcagtg tcccatcgtc ttatgcggat
1501ggcggggaaa ggcctccatt cgaacttttg tgcccaagaa tgaacggctt cattatctca
1561ggatgatggg gctggaggta ttgggagaaa agaagaagga aggggttatc ctcacaaatg
1621agagtgcagc cagcaccgga cagccagaca atgacgtgac tgagggacag agagcaggag
1681agcccaacag cccagatgca gaagaggcca acagtccaga cgtgacagca ggctgtgacc
1741cggcgggggt ccatccaccc cggtgcccaa ctttcttgta caaagtggtt ggtaagccta
1801tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc
1861gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)

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