Construct: ORF ccsbBroad304_12255
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006931.1_s304c1, BRDN0000406930
- Derived from:
- ccsbBroadEn_12255
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IARS2 (55699)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12255
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55699 | IARS2 | isoleucyl-tRNA synthetase 2... | NM_018060.4 | 69.1% | 69.1% | 1_936del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2166
- ORF length:
- 2100
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc tgaatcaaag tatgctgttg tgaaatgttc taagtctgga gacctctacg 121 tactggcggc agataaagta gcatctgttg cttctacttt ggaaacaaca tttgagacta 181 tttcaacact ttcaggtgta gatttggaaa atggtacttg cagtcatcca ttaattcctg 241 ataaagcctc tcctctttta cctgcaaatc atgtgaccat ggcaaaagga acgggattgg 301 ttcacacagc cccagctcat ggtatggaag actacggtgt agcgtctcag cacaacctgc 361 ccatggattg tctagtggac gaagatggag ttttcacaga tgttgcaggt cctgaacttc 421 aaaacaaggc tgtccttgaa gagggaactg atgtggttat aaagatgctt cagactgcaa 481 agaatttgtt gaaagaggag aaattggtgc atagctatcc gtatgactgg aggaccaaga 541 aacctgtggt tattcgtgcc agcaagcagt ggtttataaa catcacggat attaagactg 601 cagccaagga attgttaaaa aaggtgaaat ttattcctgg atcagcactg aatggcatgg 661 ttgaaatgat ggacaggcgg ccatattggt gtatatcaag gcaaagagtt tggggtgttc 721 caattcctgt gtttcatcat aagaccaagg atgaatactt gatcaacagc caaaccactg 781 agcatattgt taaactagtg gaacaacacg gcagtgatat ctggtggact cttccccctg 841 aacaacttct tccaaaagaa gtcttatctg aggttggtgg ccctgatgcc ttggaatatg 901 tgccaggtca ggatattttg gacatctggt ttgatagcgg aacttcatgg tcttatgttc 961 ttccaggtcc tgaccaaaga gcagatttgt acttggaagg aaaagaccag ctcgggggtt 1021 ggtttcagtc atccttatta acaagtgtgg cagcaaggaa gagagcacct tataagacag 1081 tgattgttca tggatttacc cttggagaaa agggagaaaa gatgtccaag tctcttggga 1141 atgtcattca tcctgatgtt gtcgttaatg gaggacaaga tcaaagcaaa gagcctccgt 1201 atggtgctga tgtccttcgc tggtgggtag ctgattccaa tgtcttcacc gaagttgcaa 1261 ttggcccatc cgtgctcaat gctgccagag atgatattag caagcttagg aatacacttc 1321 gctttctttt gggaaatgtg gctgatttca acccagaaac agattccatc cctgtaaacg 1381 atatgtatgt catagaccag tacatgctac acttactgca ggatttggca aacaagatta 1441 ccgaattata caaacaatat gattttggaa aagttgttcg gctgttacgg acgttttata 1501 ccagagagct ctctaacttt tatttcagta taatcaaaga taggctctat tgtgaaaagg 1561 aaaatgaccc caaacgacgc tcttgtcaga ctgcattagt tgaaattttg gatgtaatag 1621 ttcgttcttt tgctcccatt cttcctcacc tggctgaaga ggtgttccag cacatacctt 1681 atattaaaga gcccaagagt gttttccgta ctgggtggat tagtactagt tctatctgga 1741 aaaagcccgg gttggaagaa gctgtggaga gtgcgtgtgc aatgcgagac tcatttcttg 1801 gaagcatccc tggcaaaaat gcagctgagt acaaggttat cactgtgata gaacctggac 1861 tgctttttga gataatagag atgctgcagt ctgaagagac ttccagcacc tctcagttga 1921 atgaattaat gatggcttct gagtcaactt tactggctca ggaaccacga gagatgactg 1981 cagatgtaat cgagcttaaa gggaaattcc tcatcaactt agaaggtggt gatattcgtg 2041 aagagtcttc ctataaagta attgtcatgc cgactacgaa agaaaaatgc ccccgttgtt 2101 ggaagtatac agcggagtct tcagatacac tgtgtcctcg atgtgcagaa gttgtcagtg 2161 gaaaataccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 2221 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 2281 acctctggat ta