Construct: ORF ccsbBroad304_12350
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008351.1_s304c1, BRDN0000402466
- Derived from:
- ccsbBroadEn_12350
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NLN (57486)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12350
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57486 | NLN | neurolysin | XM_005248559.3 | 80.2% | 68.2% | (many diffs) |
| 2 | human | 57486 | NLN | neurolysin | NM_020726.5 | 79.6% | 70.6% | (many diffs) |
| 3 | human | 57486 | NLN | neurolysin | XM_017009673.1 | 76.9% | 65.1% | (many diffs) |
| 4 | human | 57486 | NLN | neurolysin | XM_006714661.2 | 76.4% | 67.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1887
- ORF length:
- 1821
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat cgcccggtgc cttttggctg tgcgaagcct ccgcagagtt ggtggttcca 121 ggattttact cagaatgacg ttaggaagag aagtgatgtc tcctcttcag gcaatgtctt 181 cctatactgt ggctggcaga aatgttttaa gatgggatct ttcaccagag caaattaaaa 241 caagaactga ggagctcatt gtgcagacca aacaggtgta cgatgctgtt ggaatgctcg 301 gtattgagga agtaacttac gagaactgtc tgcaggcact ggcagatgta gaagtaaagt 361 atatagtgga aaggaccatg ctagactttc cccagcatgt atcctctgac aaagaagtac 421 gagcagcaag tacagaagca gacaaaagac tttctcgttt tgatattgag atgagcatga 481 gaggagatat atttgagaga attgttcatt tacaggaaac ctgtgatctg gggaagataa 541 aacctgaggc cagacgatac ttggaaaagt caattaaaat ggggaaaaga aatgggctcc 601 atcttcctga acaagtacag aatgaaatca aatcaatgaa gaaaagaatg agtgagctat 661 gtattgattt taacaaaaac ctcaatgagg atgatacctt ccttgtattt tccaaggctg 721 aacttggtgc tcttcctcat gatttcattg acagtttaga aaagacagat gatgacaagt 781 ataaaattac cttaaaatat ccacactatt tccctgtcat gaagaaatgt tgtatccctg 841 aaaccagaag aaggatggaa atggctttta atacaaggtg caaagaggaa aacaccataa 901 ttttgcagca gctactccca ctgcgaacca aggtggccaa actactcggt tatagcacac 961 atgctgactt cgtccttgaa atgaacactg caaagagcac aagccgcgta acagcctttc 1021 tagatgattt aagccagaag ttaaaaccct tgggtgaagc agaacgagag tttattttga 1081 atttgaagaa aaaggaatgc aaagacaggg gttttgaata tgatgggaaa atcaatgcct 1141 gggatctata ttactacatg actcagacag aggaactcaa gtattccata gaccaagagt 1201 tcctcaagga atacttccca attgaggtgg tcactgaagg cttgctgaac acctaccagg 1261 agttgttggg actttcattt gaacaaatga cagatgctca tgtttggaac aagagtgtta 1321 cactttatac tgtgaaggat aaagctacag gagaagtatt gggacagttc tatttggacc 1381 tctatccaag ggaaggaaaa tacaatcatg cggcctgctt cggtctccag cctggctgcc 1441 ttctgcctga tggaagccgg atgatggcag tggctgccct cgtggtgaac ttctcacagc 1501 cagtggcagg tcgtccctct ctcctgagac acgacgaggt gaggacttac tttcatgagt 1561 ttggtcacgt gatgcatcag atttgtgcac aggtgagttt tttttttccc ccagtaaacc 1621 tgccaattag tttttttaga aaacttcttg actgctgtca ggtttctgct cctaacaggt 1681 tttttcagaa gctgaatgtg ttcaagaatt ttataggcct tctgcaaaac caaaattttc 1741 cttatcctgt cctctttcac aacatatttt tacttggagg ttcatctacc agtgaaagat 1801 gtttgttgaa taacagccta tggaaaaaga ataagactat ctgtaaggaa aggttacaaa 1861 tactttgctt tttcctttta atcctttacc caactttctt gtacaaagtg gttggtaagc 1921 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 1981 ggcgcgttaa gtcgacaatc aacctctgga tta