Construct: ORF ccsbBroad304_12490

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013357.1_s304c1, BRDN0000399377
Derived from:
ccsbBroadEn_12490
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AEN (64782)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12490
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64782 AEN apoptosis enhancing nuclease NM_022767.4 64.1% 64% 1_348del;418A>G;642G>C
2 human 64782 AEN apoptosis enhancing nuclease XM_005254966.1 64.1% 64% 1_348del;418A>G;642G>C
3 human 64782 AEN apoptosis enhancing nuclease XM_005254967.1 64.1% 64% 1_348del;418A>G;642G>C
4 human 64782 AEN apoptosis enhancing nuclease XM_011521905.2 64.1% 64% 1_348del;418A>G;642G>C
5 human 64782 AEN apoptosis enhancing nuclease XM_017022489.1 64.1% 64% 1_348del;418A>G;642G>C
6 human 64782 AEN apoptosis enhancing nuclease XM_006720645.3 40.1% 40% (many diffs)
7 human 64782 AEN apoptosis enhancing nuclease XM_017022490.1 40.1% 40% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
693
ORF length:
627
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt gggcacggga ccccgagggc gggtaagcga gctggcccgc tgttccattg
121tgagctacca tggcgatgtc ctctatgaca agtacatcag gcctgagatg cccatcgctg
181actaccgtac ccgctggagt ggcatcactc ggcagcacat gcgcaaggct gtccccttcc
241aggtggccca gaaagagatc cttaagctcc tgaagggcaa ggtggtggtg gggcacgcgc
301tgcacaacga cttccaggcg ctcaagtatg tccaccctcg gagccagacc cgggatacca
361cctatgtccc aaacttcctc agcgagcccg gcctccacac ccgggcccgg gtctctctaa
421aggacctggc cctgcagctg ctgcacaaga agatccaggt gggccagcac gggcactcat
481cagtagaaga tgccacgaca gccatggagc tctaccggct ggtggaggtg cagtgggaac
541agcaggaggc ccgcagcctc tggacctgcc ccgaggacag agaacctgac agcagcacag
601acatggaaca gtacatggag gaccagtact ggcccgatga cctggcccac ggcagcagag
661gaggagccag ggaggcacag gacagaagga attgcccaac tttcttgtac aaagtggttg
721gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
781accggtggcg cgttaagtcg acaatcaacc tctggatta

Download FASTA (ORF) (Full)