Construct: ORF ccsbBroad304_12552
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015910.1_s304c1, BRDN0000404487
- Derived from:
- ccsbBroadEn_12552
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FA2H (79152)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12552
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79152 | FA2H | fatty acid 2-hydroxylase | XM_011523319.2 | 39.9% | 38% | (many diffs) |
| 2 | human | 79152 | FA2H | fatty acid 2-hydroxylase | NM_024306.5 | 31.3% | 29.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 420
- ORF length:
- 354
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc caagcctctc catctgcagg cacccttcga cggctcccgc ctggtcttcc 121 cccctgtgcc agcctccctg gtgatcggcg tcttctactt gtgcatgcag ctcatcctgc 181 ctgaggcagt agggggcact gtgtttgcgg ggggcctcct gggctacgtc ctctatgaca 241 tgacccatta ctacctgcac tttggctcgc cgcacaaggg ctcctacctg tacagcctga 301 aggcccacca cgtcaagcac cactttgcac atcagaagtc aggatttggt atcagcacta 361 aattgtggga ttactgtttc cacaccctca ctccagagaa accccacctg aagacgcagt 421 gtccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 481 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 541 ggatta