Construct: ORF ccsbBroad304_12620
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014432.1_s304c1, BRDN0000401790
- Derived from:
- ccsbBroadEn_12620
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HAND2-AS1 (79804)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12620
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79804 | HAND2-AS1 | HAND2 antisense RNA 1 | NR_136198.1 | 9.6% | 1_185del;588_4177del | |
| 2 | human | 79804 | HAND2-AS1 | HAND2 antisense RNA 1 | NR_136192.1 | 9.3% | 1_297del;700_4289del | |
| 3 | human | 79804 | HAND2-AS1 | HAND2 antisense RNA 1 | NR_136197.1 | 9.2% | 1_368del;771_4360del | |
| 4 | human | 79804 | HAND2-AS1 | HAND2 antisense RNA 1 | NR_136194.1 | 9% | 1_464del;867_4456del | |
| 5 | human | 79804 | HAND2-AS1 | HAND2 antisense RNA 1 | NR_136199.1 | 8.9% | 1_507del;910_4499del | |
| 6 | human | 79804 | HAND2-AS1 | HAND2 antisense RNA 1 | NR_136193.1 | 8% | 1_294del;400_1099del;1397_4986del | |
| 7 | human | 79804 | HAND2-AS1 | HAND2 antisense RNA 1 | NR_136200.1 | 8% | 1_297del;403_1102del;1400_4989del | |
| 8 | human | 79804 | HAND2-AS1 | HAND2 antisense RNA 1 | NR_136201.1 | 7.7% | 1_464del;570_1269del;1567_5156del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 471
- ORF length:
- 402
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggatgtat aaccgaaacc agtcctctcc accgcctggg gatcttcact 121 ttcgcagtct acgactgcct gtgactccag aaagacaaac tgcagattgg ccaagctgcc 181 tgaagaccca gcccgacagc gatgcctgtc agcccgcgtc gcccaccagg gcagctgctc 241 tcccaactcg catggggggc accaccccgc ctcggtgccc tagagctgag cgcagccgcg 301 gctccacggg gatcgccagg gcctcagccc tcgcggccgg cggcgcggga gtgttgcggg 361 gcagggacca gagcgcgatc agagcggcca cccccgattt aggtcgccag ctttccagtc 421 actgcgatgg ccactggggc gccccttcca tcctggttaa attctcattg ttgccaactt 481 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 541 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta