Construct: ORF ccsbBroad304_12700

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017139.1_s304c1, BRDN0000402233
Derived from:
ccsbBroadEn_12700
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACAD10 (80724)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12700
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 80724 ACAD10 acyl-CoA dehydrogenase fami... NM_025247.6 22.3% 22.2% 1_144del;852_854delCCC;858_3177delinsA
2 human 80724 ACAD10 acyl-CoA dehydrogenase fami... NM_001136538.2 21.7% 21.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
777
ORF length:
711
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg cggagttctc attccttctc cagggagagt cgctgcagaa tgggaggtac
121agaatcgtat cccttctgga actatattaa aggccttgat ggaaggtggt gaaaatgggc
181cctggatgag atttatgaga gcagaaataa cagcagaggg ttttttacga gaatttggga
241gactttgctc tgaaatgtta aagacctccg tgcctgtgga ctcatttttc tctctgttga
301ccagtgagcg agtggcaaag cagttcccag tgatgactga ggccataact caaattcggg
361caaaaggtct tcagactgca gtcttgagca ataattttta tcttcccaac cagaaaagct
421ttttgcccct ggaccggaaa cagtttgatg tgattgtgga gtcctgcatg gaagggatct
481gtaagccaga ccctaggatc tacaagctgt gcttggagca gctcggcctg cagccctctg
541agtccatctt tcttgatgac cttggaacaa atctaaaaga agctgccaga cttggtattc
601acaccattaa ggttaatgac ccagagactg cagtaaagga attagaagct ctcttgggtt
661ttacattgag agtaggtgtt ccaaacactc ggcctgtgaa aaagacgatg gaaattccga
721aagattcctt gcagaagtac ctcaaagact tactgggtat ccagaccaca ggattatgcc
781caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
841ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
901tta

Download FASTA (ORF) (Full)