Construct: ORF ccsbBroad304_12953
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013569.1_s304c1, BRDN0000402854
- Derived from:
- ccsbBroadEn_12953
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AP5B1 (91056)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12953
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91056 | AP5B1 | adaptor related protein com... | NM_138368.5 | 25.3% | 25.3% | 1_1965del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 735
- ORF length:
- 669
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt gcaggaggca ccggccctgg tacggctgag cctggggtcc catcgggtca 121 agggcccact cccagtgttg aagctccagc cggaggcgct ggagcccatc tactctctgg 181 agctgcgctt ccgtgtggaa ggacagctgt atgcacccct ggaggctgtc catgtgccct 241 gcctgtgtcc tggccgccct gcccgccctc tgctcctgcc tctgcagccc cgatgcccgg 301 cccccgcacg gctggatgtc catgcccttt acaccacatc cactggtctc acgtgccatg 361 cccacttgcc acccctgttc gtgaactttg ccgacctctt tctgcctttc ccgcagcctc 421 cagagggggc cgggctgggc ttctttgagg agctctggga ttcctgcctg ccagagggtg 481 ctgagagtcg tgtgtggtgt ccacttgggc cacagggcct ggagggcttg gtgtcccgcc 541 acctggagcc ttttgtggtg gtggcccagc ctcctaccag ctactgtgta gcaatccacc 601 tgcccccgga ctcaaagctg ctgctgcggc tggaggcggc cctggcagat ggagtgcctg 661 tggccctgcg gaccgatgac tgggccgtgc tgcccctggc gggggactac ctccgtgggc 721 tggcggctgc tgtctgccca actttcttgt acaaagtggt tggtaagcct atccctaacc 781 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 841 cgacaatcaa cctctggatt a