Construct: ORF ccsbBroad304_12954
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005584.1_s304c1, BRDN0000408679
- Derived from:
- ccsbBroadEn_12954
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AP5B1 (91056)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12954
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91056 | AP5B1 | adaptor related protein com... | NM_138368.5 | 62.8% | 62.8% | 1_978del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1725
- ORF length:
- 1656
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcttgcgctc aaggcggcct ttggtgaggc cttgttcaca gcccaggatg 121 aagcgttgct gctccgccgg ctcaccttgg ctgcccagca ccctgctctg cctccgccca 181 cccatctctt ttaccttcac tgcgtcctga gcttccctga gaactggccg ctgggccctg 241 aaggtgagga ggctgcccca ctgctgctag ggccccagct atgccgtggt ctcctgccca 301 gtctcctgca tgacccaatg gccctcctgg cccgcctgca tttactgtgc ctgctctgtg 361 ccgaggagga agaagaggag aaaggccagc ttccaagccc acggcactac ctggaagagc 421 tgctggctgg cttgcggcag cgggcagccc tggatggggg cccccgggcc ttggccactc 481 tctgcttcca ggcctcgtat ctggtggcct gctgcctggc tgggcaacct acggtgctga 541 cccccttgat ccacggactg gcccagctgt accaagcccg gcccatgctg gctccccact 601 ttgtggacct cttggatcag gtggactctg agctgaggga gcccctgaag gtggtgttgc 661 ggcaggtggt ggtgtccagg ccgggcaggg atgaagctct ttgctggcac ctgcaaatgc 721 tggcaaaggt ggcagatgga gatgcccaga gtgctaccct caactttcta caggccgcgg 781 ctgcccactg cacgaactgg gacctacagc agggcctgct gcgggtctgc cgggcgctgc 841 tgcgggcagg ggtgaggggc ggcctggtcg acttgctgca ggtgctggcc aggcagctgg 901 aggaccctga tgggcgtgac cacgcccgcc tctactacat cctgctggca cacctggcag 961 cacccaagtt gggggtggcc ctgggcccct cgcttgccgc acctgcactg gcctcttcac 1021 tggtggccga gaaccagggc tttgtggcag cactgatggt gcaggaggca ccggccctgg 1081 tacggctgag cctggggtcc catcgggtca agggcccact cccagtgttg aagctccagc 1141 cggaggcgct ggagcccatc tactctctgg agctgcgctt ccgtgtggaa ggacagctgt 1201 atgcacccct ggaggctgtc catgtgccct gcctgtgtcc tggccgccct gcccgccctc 1261 tgctcctgcc tctgcagccc cgatgcccgg cccccgcacg gctggatgtc catgcccttt 1321 acaccacatc cactggtctc acgtgccatg cccacttgcc acccctgttc gtgaactttg 1381 ccgacctctt tctgcctttc ccgcagcctc cagagggggc cgggctgggc ttctttgagg 1441 agctctggga ttcctgcctg ccagagggtg ctgagagtcg tgtgtggtgt ccacttgggc 1501 cacagggcct ggagggcttg gtgtcccgcc acctggagcc ttttgtggtg gtggcccagc 1561 ctcctaccag ctactgtgta gcaatccacc tgcccccgga ctcaaagctg ctgctgcggc 1621 tggaggcggc cctggcagat ggagtgcctg tggccctgcg gaccgatgac tgggccgtgc 1681 tgcccctggc gggggactac ctccgtgggc tggcggctgc tgtcttgcca actttcttgt 1741 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 1801 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a