Construct: ORF ccsbBroad304_12971
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015579.1_s304c1, BRDN0000408448
- Derived from:
- ccsbBroadEn_12971
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RFT1 (91869)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12971
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91869 | RFT1 | RFT1 homolog | XR_001740360.2 | 8.5% | 1_3115del;3488_4340del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 441
- ORF length:
- 372
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatggtccc atagggtcaa gtggagcaca cacggaaggc agtcctcctt 121 ccctcccatg tcctttctct gtccctggca tccacagaaa gggaccttca gtcctcatga 181 gctgcttctc tgcagctgct gatcaccctc ccggaaccct gcacctatcc cgtgcccacc 241 tgaccccaga ctcaagcatg atcctcattc tacctgcctc ggaatcacct ggattgcaag 301 tcagaacatg gcttcccagg acccactcca ggccttcagg gtctgtgtct cccaggagga 361 gggctctgag attggctttt ctggcaagcc cacgtggctt tgatgccacc aaggttggca 421 tgccccattg tcttccttcg ttgccaactt tcttgtacaa agtggttggt aagcctatcc 481 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 541 ttaagtcgac aatcaacctc tggatta