Construct: ORF ccsbBroad304_13011
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005206.1_s304c1, BRDN0000460249
- Derived from:
- ccsbBroadEn_13011
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EGLN3 (112399)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13011
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112399 | EGLN3 | egl-9 family hypoxia induci... | NM_001308103.2 | 82% | 82% | 1_78del |
| 2 | human | 112399 | EGLN3 | egl-9 family hypoxia induci... | NM_022073.4 | 49.7% | 49.7% | 1_360del |
| 3 | mouse | 112407 | Egln3 | egl-9 family hypoxia-induci... | NM_028133.2 | 44.6% | 48.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 426
- ORF length:
- 357
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtggcttgc tatccgggaa atggaacagg ttatgttcgc cacgtggaca 121 accccaacgg tgatggtcgc tgcatcacct gcatctacta tctgaacaag aattgggatg 181 ccaagctaca tggtgggatc ctgcggatat ttccagaggg gaaatcattc atagcagatg 241 tggagcccat ttttgacaga ctcctgttct tctggtcaga tcgtaggaac ccacacgaag 301 tgcagccctc ttacgcaacc agatatgcta tgactgtctg gtactttgat gctgaagaaa 361 gggcagaagc caaaaagaaa ttcaggaatt taactaggaa aactgaatct gccctcactg 421 aagacttgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 481 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 541 acctctggat ta