Construct: ORF ccsbBroad304_13138

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009770.1_s304c1, BRDN0000407828
Derived from:
ccsbBroadEn_13138
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DRAM2 (128338)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13138
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 128338 DRAM2 DNA damage regulated autoph... NM_001349886.1 79.2% 74.5% (many diffs)
2 human 128338 DRAM2 DNA damage regulated autoph... NM_001349887.1 79.2% 74.5% (many diffs)
3 human 128338 DRAM2 DNA damage regulated autoph... NM_001349888.1 79.2% 74.5% (many diffs)
4 human 128338 DRAM2 DNA damage regulated autoph... NM_001349881.1 78.1% 78.1% 1_174del
5 human 128338 DRAM2 DNA damage regulated autoph... NM_001349882.1 78.1% 78.1% 1_174del
6 human 128338 DRAM2 DNA damage regulated autoph... NM_001349884.1 78.1% 78.1% 1_174del
7 human 128338 DRAM2 DNA damage regulated autoph... NM_001349885.1 78.1% 78.1% 1_174del
8 human 128338 DRAM2 DNA damage regulated autoph... NM_178454.5 78.1% 78.1% 1_174del
9 human 128338 DRAM2 DNA damage regulated autoph... XM_011540708.1 78.1% 78.1% 1_174del
10 human 128338 DRAM2 DNA damage regulated autoph... NM_001349889.1 65.3% 65.3% 0_1ins216
11 human 128338 DRAM2 DNA damage regulated autoph... NM_001349890.1 65.3% 65.3% 0_1ins216
12 human 128338 DRAM2 DNA damage regulated autoph... NM_001349891.1 65.3% 65.3% 0_1ins216
13 human 128338 DRAM2 DNA damage regulated autoph... NM_001349892.1 65.3% 65.3% 0_1ins216
14 human 128338 DRAM2 DNA damage regulated autoph... NM_001349893.1 65.3% 65.3% 0_1ins216
15 human 128338 DRAM2 DNA damage regulated autoph... NR_146301.1 29.6% 1_431del;757_758insAGTAAGTGCACTTAGC;1040_2032del
16 human 128338 DRAM2 DNA damage regulated autoph... NR_146302.1 29.1% (many diffs)
17 human 128338 DRAM2 DNA damage regulated autoph... NR_146308.1 27.4% 1_597del;923_924insAGTAAGTGCACTTAGC;1206_2198del
18 human 128338 DRAM2 DNA damage regulated autoph... NR_146303.1 26.9% 1_642del;968_969insAGTAAGTGCACTTAGC;1251_2243del
19 human 128338 DRAM2 DNA damage regulated autoph... NR_146305.1 25.2% (many diffs)
20 human 128338 DRAM2 DNA damage regulated autoph... NR_146306.1 25% (many diffs)
21 human 128338 DRAM2 DNA damage regulated autoph... NR_146307.1 24.7% (many diffs)
22 human 128338 DRAM2 DNA damage regulated autoph... NR_146304.1 24.5% (many diffs)
23 mouse 67171 Dram2 DNA-damage regulated autoph... XM_006501928.1 88.8% 85.6% (many diffs)
24 mouse 67171 Dram2 DNA-damage regulated autoph... NM_001286986.1 69.5% 67% (many diffs)
25 mouse 67171 Dram2 DNA-damage regulated autoph... NM_001286987.1 69.5% 67% (many diffs)
26 mouse 67171 Dram2 DNA-damage regulated autoph... NM_026013.3 69.5% 67% (many diffs)
27 mouse 67171 Dram2 DNA-damage regulated autoph... NM_001025582.2 41.1% 40.8% (many diffs)
28 mouse 67171 Dram2 DNA-damage regulated autoph... XM_006501929.3 41.1% 40.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
690
ORF length:
624
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct aaatattgcg gcagttttat gcattgctac catttatgtt cgttataagc
121aagttcatgc tctgagtcct gaagagaacg ttatcatcaa attaaacaag gctggccttg
181tacttggaat actgagttgt ttaggacttt ctattgtggc aaacttccag aaaacaaccc
241tttttgctgc acatgtaagt ggagctgtgc ttacctttgg tatgggctca ttatatatgt
301ttgttcagac catcctttcc taccaaatgc agcccaaaat ccatggcaaa caagtcttct
361ggatcagact gttgttggtt atctggtgtg gagtaagtgc acttagcatg ctgacttgct
421catcagtttt gcacagtggc aattttggga ctgatttaga acagaaactc cattggaacc
481ccgaggacaa aggttatgtg cttcacatga tcactactgc agcagaatgg tctatgtcat
541tttccttctt tggttttttc ctgacttaca ttcgtgattt tcagaaaatt tctttacggg
601tggaagccaa tttacatgga ttaaccctct atgacactgc accttgccct attaacaatg
661aacgaacacg gctactttcc agagatattt gcccaacttt cttgtacaaa gtggttggta
721agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
781ggtggcgcgt taagtcgaca atcaacctct ggatta

Download FASTA (ORF) (Full)

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