Construct: ORF ccsbBroad304_13157
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013855.1_s304c1, BRDN0000399722
- Derived from:
- ccsbBroadEn_13157
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC148 (130940)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13157
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 130940 | CCDC148 | coiled-coil domain containi... | NM_001301685.1 | 99.7% | 99.6% | 469A>G;720G>A |
| 2 | human | 130940 | CCDC148 | coiled-coil domain containi... | NM_138803.4 | 42.9% | 42.9% | 469A>G;720G>A;765_1773delinsG |
| 3 | human | 130940 | CCDC148 | coiled-coil domain containi... | XM_017003392.1 | 42% | 42% | 469A>G;720G>A;765_1812delinsG |
| 4 | human | 130940 | CCDC148 | coiled-coil domain containi... | XM_005246319.3 | 41.5% | 41.5% | (many diffs) |
| 5 | human | 130940 | CCDC148 | coiled-coil domain containi... | XM_011510638.3 | 40.6% | 40.6% | (many diffs) |
| 6 | human | 130940 | CCDC148 | coiled-coil domain containi... | XM_017003394.1 | 33.2% | 33.2% | (many diffs) |
| 7 | human | 130940 | CCDC148 | coiled-coil domain containi... | XM_017003396.1 | 28.4% | 28.4% | (many diffs) |
| 8 | human | 130940 | CCDC148 | coiled-coil domain containi... | XM_024452710.1 | 28.4% | 28.4% | (many diffs) |
| 9 | human | 130940 | CCDC148 | coiled-coil domain containi... | XM_017003395.1 | 27.8% | 27.8% | (many diffs) |
| 10 | human | 130940 | CCDC148 | coiled-coil domain containi... | NM_001301684.1 | 18.3% | 16.4% | (many diffs) |
| 11 | human | 130940 | CCDC148 | coiled-coil domain containi... | XM_017003397.1 | 18.3% | 16.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 831
- ORF length:
- 765
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtg tgcagcttct gcttctccag ataatctggt attccatatg aaaaatgaga 121 tgagaaacat caagtacaaa ccagtagact atcaacaatt gcgtgcatta actgaagcaa 181 agaaattggc ttctgcctct gcaaagctaa agatcagaaa agcaatgttg acttcaaagt 241 tatccaaaga acaaacacta ataaaacaac acaagcaagt gtggtggcag gaataccaga 301 ggctgaatga agtcagatgt aaaatggaat ctgaaataaa atcccttctc aatgaagaga 361 acattggaaa tgaatgtctt tgtgacctta caaattttga gcaagagcta tcagaacaac 421 agtgcacata tcttaaaaat gtaataaatc ctattcagca gctgagagca gatctaaaat 481 acagacagca tcacactttg cagcattcac acccacatat tgagtttaac tccgtgaaag 541 tattggaaga ggttgacttt gtgaagaaac aattgaaaac tgtctttgaa agacttaggc 601 tggagcaaca gagaatagaa aatgatcttt cagactggag tataaagatt ctagatcatt 661 ctttggaaga aaagactaac ccgcttagtg aactgcccat tgaattagaa agtttggaat 721 gcccataccc tgatttgaaa tcttcaattc tcagtgagtt ctataagttt acacagaaat 781 atcaaaagaa gcttcaagac tttaatctgc agttggaaga catatacagg tgcccaactt 841 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 901 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta