Construct: ORF ccsbBroad304_13360
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009672.1_s304c1, BRDN0000399233
- Derived from:
- ccsbBroadEn_13360
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DHX36 (170506)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13360
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 170506 | DHX36 | DEAH-box helicase 36 | NM_020865.3 | 97% | 96.9% | 1247C>G;1748T>A;2205_2291del |
| 2 | human | 170506 | DHX36 | DEAH-box helicase 36 | NM_001114397.1 | 95.6% | 95.5% | (many diffs) |
| 3 | mouse | 72162 | Dhx36 | DEAH (Asp-Glu-Ala-His) box ... | NM_028136.2 | 85.6% | 88.9% | (many diffs) |
| 4 | mouse | 72162 | Dhx36 | DEAH (Asp-Glu-Ala-His) box ... | XM_011240238.1 | 85.6% | 88.9% | (many diffs) |
| 5 | mouse | 72162 | Dhx36 | DEAH (Asp-Glu-Ala-His) box ... | XM_011240240.2 | 62.1% | 61.6% | (many diffs) |
| 6 | mouse | 72162 | Dhx36 | DEAH (Asp-Glu-Ala-His) box ... | XM_011240242.2 | 53.5% | 55.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3003
- ORF length:
- 2937
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag ttatgactac catcagaact ggggccgtga tgggggtccc cgcagctccg 121 gtgggggcta tggagggggg ccagcagggg gtcatggagg taaccgaggc tccggaggag 181 gcggcggcgg cggagggggt ggtcgaggcg gcaggggccg gcatcccggg cacctgaaag 241 gccgcgaaat cggcatgtgg tacgcgaaaa aacaggggca gaagaacaag gaagcggaga 301 ggcaagagag agctgtagta cacatggatg aacgacgaga agaacaaatt gtacagttac 361 tgaattctgt tcaagcgaag aatgataaag agtcagaagc acagatatcc tggtttgctc 421 ctgaggatca tggatacggt actgaagttt ctactaagaa cacaccatgc tcagagaaca 481 aacttgacat ccaggaaaag aagttgataa atcaagaaaa aaaaatgttt agaatcagga 541 acagatcata tattgaccga gattctgagt atctcttgca agaaaatgaa ccagatggaa 601 ctttagacca aaaattattg gaagatttac aaaagaaaaa aaatgacctt cggtatattg 661 aaatgcagca tttcagagaa aagctgcctt cgtatggaat gcaaaaggaa ttggtaaatt 721 taattgataa ccatcaggta acagtaataa gtggtgaaac tggttgtggc aaaaccactc 781 aagttactca gttcattttg gataactaca ttgaaagagg aaaaggatct gcttgcagaa 841 tagtttgtac tcagccaaga agaattagtg ccatttcagt tgcggaaaga gtagctgcag 901 aaagggcaga atcttgtggc agtggtaata gtactggata tcaaattcgt ctccagagtc 961 ggttgccaag gaaacagggt tctatcttat actgtacaac aggaatcatc cttcagtggc 1021 tccagtcaga cccgtatttg tccagtgtta gtcatatcgt acttgatgaa atccatgaaa 1081 gaaatctgca gtcagatgtt ttaatgactg ttgttaaaga ccttctcaat tttcgatctg 1141 acttgaaagt aatattgatg agtgcaacat tgaatgcaga aaagttttca gaatattttg 1201 gtaactgtcc aatgatacat atacctggtt ttacctttcc ggttgtggaa tatcttttgg 1261 aagatgtaat tgaaaaaata aggtatgttc cagaacaaaa agaacacaga tgccagttta 1321 agaggggttt catgcaaggg catgtaaata gacaagaaaa agaagaaaaa gaagcaatat 1381 ataaagaacg ttggccagat tatgtaaggg aactgcgaag aaggtattct gcaagtactg 1441 tagatgttat agaaatgatg gaggatgata aagttgatct gaatttgatt gttgccctca 1501 tccgatacat tgttttggaa gaagaggatg gtgcgatact ggtctttctg ccaggctggg 1561 acaatatcag cactttacat gatctcttga tgtcacaagt aatgtttaaa tcagataaat 1621 ttttaattat acctttacat tcactgatgc ctacagttaa ccagacacag gtgtttaaaa 1681 gaacccctcc tggtgttcgg aaaatagtaa ttgctaccaa cattgcggag actagcatta 1741 ccatagatga tgtcgtttat gtgatagatg gaggaaaaat aaaagagacg cattttgata 1801 ctcagaacaa taacagtaca atgtccgctg agtgggttag taaagctaat gccaaacaga 1861 gaaaaggtcg agctggaaga gttcaacctg gtcattgcta tcatctgtat aatggtctta 1921 gagcaagtct tctagatgac tatcaactgc cagaaatttt gagaactcct ttggaagaac 1981 tttgtttaca aataaagatt ttaaggctag gtggaattgc ttattttctg agtagattaa 2041 tggacccacc atcaaatgag gcagtgttac tctccataag acacctgatg gagctgaacg 2101 ctttggataa acaagaagaa ttgacacctc ttggagtcca cttggcacga ttacccgttg 2161 agccacatat tggaaaaatg attctttttg gagcactgtt ctgctgctta gacccagtac 2221 tcactattgc tgctagtctc agtttcaaag atccatttgt cattccactg ggctgggaag 2281 aggctaggcg acgtggtttc agatacgaaa aggactattg ctgggaatat tttctgtctt 2341 caaacacact gcagatgctg cataacatga aaggacagtt tgctgagcat cttcttggag 2401 ctggatttgt aagcagtaga aatcctaaag atccagaatc taatataaat tcagataatg 2461 agaagataat taaagctgtc atctgtgctg gtttatatcc caaagttgct aaaattcgac 2521 taaatttggg taaaaaaaga aaaatggtaa aagtttacac aaaaaccgat ggcctggttg 2581 ctgttcatcc taaatctgtt aatgtggagc aaacagactt tcactacaac tggcttatct 2641 atcacctaaa gatgagaaca agcagtatat acttgtatga ctgcacagag gtttccccat 2701 actgtctctt gttttttgga ggtgacattt ccatccagaa ggataacgat caggaaacta 2761 ttgctgtaga tgagtggatt gtatttcagt ctccagcaag aattgcccat cttgttaagg 2821 aattaagaaa ggaactagat attcttctgc aagagaagat tgaaagtcct catcctgtag 2881 actggaatga cactaaatcc agagactgtg cagtactgtc agctattata gacttgatca 2941 aaacacagga aaaggcaact cccaggaact ttccgccacg attccaggat ggatattaca 3001 gctgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 3061 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 3121 tctggatta