Construct: ORF ccsbBroad304_13363
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013975.1_s304c1, BRDN0000402530
- Derived from:
- ccsbBroadEn_13363
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPTSSA (171546)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13363
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 171546 | SPTSSA | serine palmitoyltransferase... | NM_138288.4 | 95.3% | 94.3% | 1_9delATGGCGGGG;166A>G |
| 2 | mouse | 104725 | Sptssa | serine palmitoyltransferase... | NM_134054.2 | 86.8% | 92.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 270
- ORF length:
- 204
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gctggcgcgg gcctggaagc agatgtcctg gttctactac cagtacctgc 121 tggtcacggc gctctacatg ctggagccct gggagcggac ggtgttcaat tccatgctgg 181 tttccattgt ggggatggca ctatacacag gatacgtctt cgtgccccag cacatcatgg 241 cgatattgca ctactttgaa atcgtacaat gcccaacttt cttgtacaaa gtggttggta 301 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 361 ggtggcgcgt taagtcgaca atcaacctct ggatta