Construct: ORF ccsbBroad304_13373

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016696.1_s304c1, BRDN0000400612
Derived from:
ccsbBroadEn_13373
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FAM227B (196951)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13373
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 196951 FAM227B family with sequence simila... NM_001330293.2 100% 100%
2 human 196951 FAM227B family with sequence simila... XM_024449863.1 92.2% 92% 912_987delinsC
3 human 196951 FAM227B family with sequence simila... XM_011521324.1 89.9% 89.9% 645_746del
4 human 196951 FAM227B family with sequence simila... XM_011521325.3 89.8% 89.6% 645_746del;1013T>G
5 human 196951 FAM227B family with sequence simila... XM_005254220.3 83.6% 83.4% 645_746del;1014_1089delinsC
6 human 196951 FAM227B family with sequence simila... XM_024449862.1 83.6% 83.4% 645_746del;1014_1089delinsC
7 human 196951 FAM227B family with sequence simila... XM_005254219.3 79.9% 80% 645_746del;1014_1140delinsC
8 human 196951 FAM227B family with sequence simila... XM_011521321.2 77.4% 77.5% 645_746del;1014_1176delinsC
9 human 196951 FAM227B family with sequence simila... XM_024449865.1 76% 76% 405_406ins141;504_605del
10 human 196951 FAM227B family with sequence simila... XM_017021995.1 75.9% 76% 645_746del;1014_1200delinsC
11 human 196951 FAM227B family with sequence simila... XM_024449864.1 70.7% 70.5% 405_406ins141;504_605del;873_948delinsC
12 human 196951 FAM227B family with sequence simila... XM_024449866.1 61.1% 61.2% 404_405ins240;672_858delinsC
13 human 196951 FAM227B family with sequence simila... XM_005254216.3 60.8% 60.9% 912_1497delinsC
14 human 196951 FAM227B family with sequence simila... XM_024449867.1 60.7% 60.6% 0_1ins249;396_497del;765_840delinsC
15 human 196951 FAM227B family with sequence simila... NM_152647.3 59.7% 59.8% 645_746del;1014_1524delinsC
16 human 196951 FAM227B family with sequence simila... XM_006720424.2 59.7% 59.8% 645_746del;1014_1524delinsC
17 human 196951 FAM227B family with sequence simila... XM_005254213.3 56.9% 57% 645_746del;1014_1599delinsC
18 human 196951 FAM227B family with sequence simila... XM_005254214.3 56.9% 57% 645_746del;1014_1599delinsC
19 human 196951 FAM227B family with sequence simila... XM_006720423.3 56.9% 57% 645_746del;1014_1599delinsC
20 human 196951 FAM227B family with sequence simila... XM_011521319.2 56.9% 57% 645_746del;1014_1599delinsC
21 human 196951 FAM227B family with sequence simila... XM_005254215.3 54.7% 54.7% 404_405ins36;609_710del;978_1563delinsC
22 human 196951 FAM227B family with sequence simila... XM_017021993.1 54.1% 54.2% 405_406ins141;771_1281delinsC
23 human 196951 FAM227B family with sequence simila... XM_017021990.1 51.4% 51.5% 405_406ins141;771_1356delinsC
24 human 196951 FAM227B family with sequence simila... XM_006720426.2 48.1% 48.2% 405_406ins141;504_605del;873_1458delinsC
25 human 196951 FAM227B family with sequence simila... XM_017021994.1 44.8% 44.8% 404_405ins240;672_1257delinsC
26 human 196951 FAM227B family with sequence simila... XM_024449861.1 44.2% 44.2% 0_1ins249;663_1248delinsC
27 human 196951 FAM227B family with sequence simila... XM_006720427.4 41.4% 41.4% 0_1ins249;396_497del;765_1350delinsC
28 human 196951 FAM227B family with sequence simila... XM_017021991.2 41.4% 41.4% 0_1ins249;396_497del;765_1350delinsC
29 human 196951 FAM227B family with sequence simila... XM_017021992.1 38.2% 38.2% 104_105ins300;345_446del;714_1299delinsC
30 human 196951 FAM227B family with sequence simila... XM_011521320.1 31.8% 31.8% 0_1ins402;243_344del;612_1197delinsC
31 human 196951 FAM227B family with sequence simila... XM_017021996.1 31.8% 31.8% 0_1ins402;243_344del;612_1197delinsC
32 human 196951 FAM227B family with sequence simila... XM_011521322.1 29.5% 29.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
978
ORF length:
912
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc aggccaacga acatgtcaaa ggaggagcag cagagctggc cccgggaaaa
121tgcaagaacc tccaaagagc attgaagaat tcttaaagtt tcaaaattgg gattattggc
181caagggaaat ccattttaga gatgatgata aatggtcatg cactctgaaa aaaataaaag
241aagatagttc atttgtttca atttatacac acctatggga aaatgttcct cgaatatttg
301aagcactttt gatcatggaa tcaaaattaa aggaatattc acttattttg caaaaccaca
361cctctgaaat atttaagtgg aaaagcatga tttctgagac aagttcttat agaaaattgg
421aacgatatgg ggaatttcta aagaagtacc ataagaaaaa aaagataatg ctttcagatg
481aaatggagac agagaagaat atagagggtt gcagcttcac aggattcaaa gcaaatgaac
541ttactcagct acccagacat ttggatgctg aacaaattta tctttttatt ttaaaagccc
601ataattttga tgaaagagtt tttaaaatct ggaagactca ttttctctca gaagcctcca
661ttgctctttt gcatgactcc ttttggtggt ggtttctcca taaatttagg atatatcctg
721attgtttggc acaagccata tatgcaacgt tccatgaagc atttccagaa tcgagttacc
781tctttaatga tgaatttaaa gaagatctag ggaataacat ttttctttgg tgttcaggtt
841taaaacctca aaaaggcttt tggatccact ggaaactgaa agaactctcc acaaccacca
901ttcatggtag caaaaaagca cctgcaaaat cagtaaagga aagaattgca gacagtcaag
961aacatatatc aactagctgc ccaactttct tgtacaaagt ggttggtaag cctatcccta
1021accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
1081agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)