Construct: ORF ccsbBroad304_13562
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012358.1_s304c1, BRDN0000404952
- Derived from:
- ccsbBroadEn_13562
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C8orf31 (286122)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13562
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 286122 | C8orf31 | chromosome 8 open reading f... | NR_149322.1 | 9.3% | (many diffs) | |
| 2 | human | 286122 | C8orf31 | chromosome 8 open reading f... | NR_126398.1 | 8.8% | 1_405del;592_2110del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 252
- ORF length:
- 186
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tgaaaagcca caccagaatt cctgcaattc agtgaggcag ctcttcaaaa 121 ccaagcagct ggtgacgcac agggacagag gggcccaggg gctgctggct gccaggacca 181 ccgcattgca gaggtctccc ttgcagcaag agatctggga gtccacaacg gcacttgttc 241 ttccaggcag gtacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 301 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 361 caatcaacct ctggatta