Construct: ORF ccsbBroad304_13616
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001462.1_s304c1, BRDN0000407667
- Derived from:
- ccsbBroadEn_13616
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EP400P1 (347918)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13616
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57634 | EP400 | E1A binding protein p400 | NM_015409.5 | 12.3% | 10.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1323
- ORF length:
- 1257
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca gcacgtcagc agctcccaga gcagccagcg ccatgtccag tggcctgggg 121 cctgccccgg cgcgggcgag gagcagccag cgtgctccca gccgtccctg cccctcacac 181 tgccatcccc cagccaccaa ctacagcagc tgatggtcca gacccagagt cccacgcagc 241 ccagtccggg gccggggcag gccttgcaga atgtgcgtgc aggtgcccct ggccctgggc 301 tgggcctctg cagcagcagc cctacagggg acttcgtgga tgccagcgtg ctggtgaggc 361 agatcagctt gagcccctcc agtggtggac actttgtgtt tcaggatggg tcagggctca 421 cccagatcgc ccagggagcc caggttcagc tccagcaccc gggtacgccc atcacagtcc 481 gagagcggag accctcccag ccccacacac agtcaggggg caccatccac cacctgggac 541 cccagagccc tgcagccgcg ggtggggccg gcctgcagcc cctggccagc ccaagccaca 601 tcaccacggc taacttgcca ccgcagatca gcagcatcat ccagggccag ctggttcagc 661 agcagcaggt gctgcagggg ccgccgctgc cccggcccct gggcttcgag aggacgcccg 721 gcgtgctgct ccccggggct gggggcgcag cggggtttgg gatgacgtcc ccacccccgc 781 ccaccagccc ttccaggact gccgtgcccc caggcctttc cagcctccca ctcacgtctg 841 tggggaacac gggaatgaag aaggttccca agaagttaga ggagattccc ccagcctctc 901 cggagatggc acagatgagg aagcagtgcc tggactatca tcaccaggag atgcaggctc 961 tgaaggaggt cttcaaggag tatttgattg aactgttttt cttgcaacac tttcaaggga 1021 acatgatgga tttcttagct ttcaaggaga gactgtatgg accattacaa gcatatctta 1081 ggcagaatga tttggacatt gaagaagagg aagaggagca ctttgaagtc attaatgatg 1141 aggtaaaggt tgtggccaga aagcacgggc agcctgggac tcctgttgcc atagcaaccc 1201 agctaccgcc gaggacttct gcggcttttc cagcccagca gcagccgctc caggtacttt 1261 ctgatggctc cacagtgcag ctccccagac tttcctcact cggatttgag gactcgatgt 1321 gctgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 1381 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 1441 tctggatta