Construct: ORF ccsbBroad304_13685
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012948.1_s304c1, BRDN0000460257
- Derived from:
- ccsbBroadEn_13685
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FER1L6-AS1 (439941)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13685
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 439941 | FER1L6-AS1 | FER1L6 antisense RNA 1 | NR_040044.1 | 17.8% | 1_177del;592_2315del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 483
- ORF length:
- 414
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacatcctc ccttaccttc acatgtcaca tggcaagtgc cctctcctgg 121 tgaggggcaa gggtgagatg gagggagaag ccctgttgag ctgcttggcc atgaacagtt 181 tgggggaaca ggaagcttgc cttgacctcg ggtccaagac tccctcccta gagatctcat 241 caaataacca ggaaagacca accaacagag aagagactgg aatcatctgc ccagaaagac 301 ttttcatcta ttcttctaag gacagctcca agagattacc tgggggactt tgtataaaaa 361 ataagacaac ctgtgttcct gtgcagcttc atccctcatc tttccctaag tgtcaagaag 421 caatagttag tccacaagca cgagtgaaat tactgaacaa aattaagatg gatacagcac 481 tcttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 541 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 601 tctggatta