Construct: ORF ccsbBroad304_13710
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015106.1_s304c1, BRDN0000404770
- Derived from:
- ccsbBroadEn_13710
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BOLA2 (552900)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13710
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 654483 | BOLA2B | bolA family member 2B | NM_001039182.4 | 66.2% | 62.7% | (many diffs) |
| 2 | human | 654483 | BOLA2B | bolA family member 2B | NM_001320607.2 | 63.6% | 41.7% | (many diffs) |
| 3 | human | 552900 | BOLA2 | bolA family member 2 | NR_135304.1 | 41% | (many diffs) | |
| 4 | human | 552900 | BOLA2 | bolA family member 2 | NM_001031827.2 | 37.5% | 35.5% | (many diffs) |
| 5 | human | 552900 | BOLA2 | bolA family member 2 | NM_001320579.1 | 32.6% | 21% | (many diffs) |
| 6 | human | 107282092 | BOLA2-SMG1P6 | BOLA2-SMG1P6 readthrough | NM_001320623.1 | 20% | 18.7% | (many diffs) |
| 7 | human | 107282092 | BOLA2-SMG1P6 | BOLA2-SMG1P6 readthrough | NM_001320622.1 | 14.2% | 9.8% | 1_198del;260_261ins38;335_915del |
| 8 | human | 107282092 | BOLA2-SMG1P6 | BOLA2-SMG1P6 readthrough | NR_135316.1 | 9.6% | (many diffs) | |
| 9 | mouse | 66162 | Bola2 | bolA-like 2 (E. coli) | NM_175103.3 | 59% | 58.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 240
- ORF length:
- 174
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga actcagcgcc gaatacctcc gcgagaagct gcagcgggac ctggaggcgg 121 agcatgtgga ggtggaggac acgaccctca accgttgctc ctgtagcttc cgagtcctgg 181 tggtgtcggc caagttcgag gggaaaccgc tgcttcagag acacaggttc tgtacagagt 241 acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 301 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 361 ggatta