Construct: ORF ccsbBroad304_13861
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007740.1_s304c1, BRDN0000408986
- Derived from:
- ccsbBroadEn_13861
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNB3 (2784)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13861
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2784 | GNB3 | G protein subunit beta 3 | NM_002075.4 | 87.2% | 87% | 539T>C;702_830del |
| 2 | human | 2784 | GNB3 | G protein subunit beta 3 | NM_001297571.2 | 86.9% | 86.7% | 494_495insGTG;536T>C;699_827del |
| 3 | human | 2784 | GNB3 | G protein subunit beta 3 | XM_011520953.3 | 77.6% | 72.5% | (many diffs) |
| 4 | mouse | 14695 | Gnb3 | guanine nucleotide binding ... | NM_013530.1 | 79% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 957
- ORF length:
- 891
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ggagatggag caactgcgtc aggaagcgga gcagctcaag aagcagattg 121 cagatgccag gaaagcctgt gctgacgtta ctctggcaga gctggtgtct ggcctagagg 181 tggtgggacg agtccagatg cggacgcggc ggacgttaag gggacacctg gccaagattt 241 acgccatgca ctgggccact gattctaagc tgctggtaag tgcctcgcaa gatgggaagc 301 tgatcgtgtg ggacagctac accaccaaca aggtgcacgc catcccactg cgctcctcct 361 gggtcatgac ctgtgcctat gccccatcag ggaactttgt ggcatgtggg gggctggaca 421 acatgtgttc catctacaac ctcaaatccc gtgagggcaa tgtcaaggtc agccgggagc 481 tttctgctca cacaggttat ctctcctgct gccgcttcct ggatgacaac aatattgtga 541 ccagctcggg ggacaccacg tgtgccttgt gggacattga gactgggcag cagaagactg 601 tatctgtggg acacacgggt gactgcatga gcctggctgt gtctcctgac ttcaatctct 661 tcatttcggg ggcctgtgat gccagtgcca agctctggga tgtgcgagag gggacctgcc 721 gtcagacttt cactggccac gagtcggaca tcaacgccat ctgtttcttc tccctcagtg 781 gccgcctact attcgctggc tacgacgact tcaactgcaa tgtctgggac tccatgaagt 841 ctgagcgtgt gggcatcctc tctggccacg ataacagggt gagctgcctg ggagtcacag 901 ctgacgggat ggctgtggcc acaggttcct gggacagctt cctcaaaatc tggaactgcc 961 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 1021 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 1081 tta