Construct: ORF ccsbBroad304_13898
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003619.1_s304c1, BRDN0000400035
- Derived from:
- ccsbBroadEn_13898
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MNDA (4332)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13898
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4332 | MNDA | myeloid cell nuclear differ... | NM_002432.3 | 99.9% | 99.5% | 1216delG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1287
- ORF length:
- 1221
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt gaatgaatac aagaaaattc ttttgctgaa aggatttgag ctcatggatg 121 attatcattt tacatcaatt aagtccttac tggcctatga tttaggacta actacaaaaa 181 tgcaagagga atacaacaga attaagatta cagatttgat ggaaaaaaag ttccaaggcg 241 ttgcctgtct agacaaacta atagaacttg ccaaagatat gccatcactt aaaaaccttg 301 ttaacaatct tcgaaaagag aagtcaaaag ttgctaagaa aattaaaaca caagaaaaag 361 ctccagtgaa aaaaataaac caggaagaag tgggtcttgc ggcacctgca cccaccgcaa 421 gaaacaaact gacatcggaa gcaagaggga ggattcctgt agctcagaaa agaaaaactc 481 caaacaaaga aaagactgaa gccaaaagga ataaggtgtc ccaagagcag agtaagcccc 541 caggtccctc aggagccagc acatctgcag ctgtggatca tcccccacta ccccagacct 601 catcatcaac tccatccaac acttcgttta ctccgaatca ggaaacccag gcccaacggc 661 aggtggatgc aagaagaaat gttccccaaa acgacccagt gacagtggtg gtactgaaag 721 caacagcgcc atttaaatac gagtccccag aaaatgggaa aagcacaatg tttcatgcta 781 cagtggccag taagactcaa tatttccatg tgaaagtctt cgacatcaac ttgaaagaga 841 aatttgtaag gaagaaggtc attaccatat ctgattactc tgaatgtaaa ggagtaatgg 901 aaataaagga agcatcatct gtgtctgact ttaatcaaaa ttttgaggtc ccaaacagaa 961 ttatcgaaat agcaaataaa actcccaaga tcagtcaact ttacaagcaa gcatctggaa 1021 caatggtgta tgggttgttt atgttacaaa agaaaagcgt acacaagaag aacacaattt 1081 atgaaataca ggataataca ggatccatgg atgtagtggg gagtggaaaa tggcacaata 1141 tcaagtgtga gaaaggagat aaacttcgac tcttctgcct tcaactgaga acagttgacc 1201 gcaagctgaa actggtgtgt ggaagtcaca gcttcatcaa ggtcatcaag gccaagaaaa 1261 acaaggaagg accaatgaat ttaattgccc aactttcttg tacaaagtgg ttggtaagcc 1321 tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg 1381 gcgcgttaag tcgacaatca acctctggat ta