Construct: ORF ccsbBroad304_13945
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012120.1_s304c1, BRDN0000402660
- Derived from:
- ccsbBroadEn_13945
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RPA3 (6119)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13945
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6119 | RPA3 | replication protein A3 | NM_002947.5 | 99.7% | 5.7% | 12delG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 114
- ORF length:
- 48
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt ggacatatgg acttgcccag gtcgcgcatc aacgccggca tgctagctca 121 attcatcgac aagcctgtct gcttcgtagg gaggctggaa aagattcatc ccaccggaaa 181 aatgtttatt ctttcagatg gagaaggaaa aaatggaacc atcgagttga tggaacccct 241 tgatgaagaa atctctggaa ttgtggaagt ggttggaaga gtaaccgcca aggccaccat 301 cttgtgtaca tcttatgtcc agtttaaaga agatagccat ccttttgatc ttggacttta 361 caatgaagct gtgaaaatta tccatgactt ccctcagttt tatcctttag ggattgtgca 421 acatgattgc ccaactttct tgtacaaagt ggttggtaag cctatcccta accctctcct 481 cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta agtcgacaat 541 caacctctgg atta