Construct: ORF ccsbBroad304_13953
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000764.1_s304c1, BRDN0000403798
- Derived from:
- ccsbBroadEn_13953
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SKIL (6498)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13953
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6498 | SKIL | SKI like proto-oncogene | NM_001248008.1 | 99.8% | 99.2% | 113C>T;792G>T;2037delA |
| 2 | human | 6498 | SKIL | SKI like proto-oncogene | NM_005414.5 | 99.8% | 99.2% | 113C>T;792G>T;2037delA |
| 3 | human | 6498 | SKIL | SKI like proto-oncogene | XM_005247721.1 | 99.8% | 99.2% | 113C>T;792G>T;2037delA |
| 4 | human | 6498 | SKIL | SKI like proto-oncogene | XM_006713735.1 | 99.8% | 99.2% | 113C>T;792G>T;2037delA |
| 5 | human | 6498 | SKIL | SKI like proto-oncogene | NM_001145098.3 | 96.9% | 96.3% | (many diffs) |
| 6 | human | 6498 | SKIL | SKI like proto-oncogene | NM_001145097.2 | 93.1% | 92.3% | (many diffs) |
| 7 | human | 6498 | SKIL | SKI like proto-oncogene | XR_001740238.1 | 78.8% | (many diffs) | |
| 8 | mouse | 20482 | Skil | SKI-like | NM_011386.3 | 84.5% | 87.7% | (many diffs) |
| 9 | mouse | 20482 | Skil | SKI-like | XM_006535427.3 | 84.5% | 87.7% | (many diffs) |
| 10 | mouse | 20482 | Skil | SKI-like | NM_001039090.2 | 78.8% | 82.1% | (many diffs) |
| 11 | mouse | 20482 | Skil | SKI-like | NM_001271772.1 | 69.1% | 71.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2118
- ORF length:
- 2052
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga aaacctccag acaaatttct ccttggttca gggctcaact aaaaaactga 121 atgggatggg agatgatggc agccccccag cgaaaaaaat gataacggac attcatgtaa 181 atggaaaaac gataaacaag gtgccaacag ttaagaagga acacttggat gactatggag 241 aagcaccagt ggaaactgat ggagagcatg ttaagcgaac ctgtacttct gttcctgaaa 301 ctttgcattt aaatcccagt ttgaaacaca cattggcaca attccattta agtagtcaga 361 gctcgctggg tggaccagca gcattttctg ctcggcattc ccaagaaagc atgtcgccta 421 ctgtatttct gcctcttcca tcacctcagg ttcttcctgg cccattgctc atcccttcag 481 atagctccac agaactcact cagactgtgt tggaagggga atctatttct tgttttcaag 541 ttggaggaga aaagagactc tgtttgcccc aagtcttaaa ttctgttctc cgagaattta 601 cactccagca aataaataca gtgtgtgatg aactgtacat atattgttca aggtgtactt 661 cagaccagct tcatatctta aaggtactgg gcatacttcc attcaatgcc ccatcctgtg 721 ggctgattac attaactgat gcacaaagat tatgtaatgc tttattgcgg ccacgaactt 781 ttcctcaaaa tggtagcgta cttcctgcta aaagctcatt ggcccagtta aaggaaactg 841 gcagtgcctt tgaagttgag catgaatgcc taggcaaatg tcagggttta tttgcacccc 901 agttttatgt tcagcctgat gctccgtgta ttcaatgtct ggagtgttgt ggaatgtttg 961 caccccagac gtttgtgatg cattctcaca gatcacctga caaaagaact tgccactggg 1021 gctttgaatc agctaaatgg cattgctatc ttcatgtgaa ccaaaaatac ttaggaacac 1081 ctgaagaaaa gaaactgaag ataattttag aagaaatgaa ggagaagttt agcatgagaa 1141 gtggaaagag aaatcaatcc aagacagatg caccatcagg aatggaatta cagtcatggt 1201 atcctgttat aaagcaggaa ggtgaccatg tttctcagac acattcattt ttacacccca 1261 gctactactt atacatgtgt gataaagtgg ttgccccaaa tgtgtcactt acttctgctg 1321 tatcccagtc taaagagctc acaaagacag aggcaagtaa gtccatatca agacagtcag 1381 agaaggctca cagtagtggt aaacttcaaa aaacagtgtc ttatccagat gtctcacttg 1441 aggaacagga gaaaatggat ttaaaaacaa gtagagaatt atgtagccgt ttagatgcat 1501 caatctcaaa taattctaca agtaaaagga aatctgagtc tgccacttgc aacttagtca 1561 gagacataaa caaagtggga attggccttg ttgctgccgc ttcatctccg cttcttgtga 1621 aagatgtcat ttgtgaggat gataagggaa aaatcatgga agaagtaatg agaacttatt 1681 taaaacaaca ggaaaaacta aacttgattt tgcaaaagaa gcaacaactt cagatggaag 1741 taaaaatgtt gagtagttca aaatctatga aggaactcac tgaagaacag cagaatttac 1801 agaaagagct tgaatctttg cagaatgaac atgctcaaag aatggaagaa ttttatgttg 1861 aacagaaaga cttagagaaa aaattggagc agataatgaa gcaaaaatgt acctgtgact 1921 caaatttaga aaaagacaaa gaggctgaat atgcaggaca gttggcagaa ctgaggcaga 1981 gattggacca tgctgaggcc gataggcaag aactccaaga tgaactcaga caggaacggg 2041 aagcaagaca gaagttagag atgatgataa aagagctaaa gctgcaaatt ctgaaatcat 2101 caagactgct aaagaatacc caactttctt gtacaaagtg gttggtaagc ctatccctaa 2161 ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt ggcgcgttaa 2221 gtcgacaatc aacctctgga tta