Construct: ORF ccsbBroad304_14015
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003130.1_s304c1, BRDN0000402544
- Derived from:
- ccsbBroadEn_14015
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATG12 (9140)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14015
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9140 | ATG12 | autophagy related 12 | NM_001277783.2 | 99.5% | 98.6% | 211T>G |
| 2 | human | 9140 | ATG12 | autophagy related 12 | NM_004707.4 | 52.6% | 42.1% | 164_300del;348T>G;360_420del |
| 3 | human | 9140 | ATG12 | autophagy related 12 | NR_033363.2 | 5.3% | (many diffs) | |
| 4 | human | 9140 | ATG12 | autophagy related 12 | NR_033362.2 | 5.3% | (many diffs) | |
| 5 | human | 9140 | ATG12 | autophagy related 12 | NR_073603.2 | 5.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 288
- ORF length:
- 222
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggaggagccg cagtctgtgt tgcagcttcc tacttcaatt gctgctggag 121 gggaaggact tacggatgtc tccccagaaa caaccacccc ggagcccccg tcttccgctg 181 cagtttcccc gggaacagag gaacctgctg gcgacaccaa gaaaaaaatt tatttatgtg 241 aatcagtcct ttgctccttc cccagaccaa gaagtgggaa ctctctatgc ccaactttct 301 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 361 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta