Construct: ORF ccsbBroad304_14057
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005928.1_s304c1, BRDN0000407069
- Derived from:
- ccsbBroadEn_14057
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PGRMC1 (10857)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14057
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10857 | PGRMC1 | progesterone receptor membr... | NM_006667.5 | 99.8% | 6.8% | 15delT |
| 2 | human | 10857 | PGRMC1 | progesterone receptor membr... | NM_001282621.1 | 73.1% | 8.7% | 15delT;328_329ins156 |
| 3 | mouse | 53328 | Pgrmc1 | progesterone receptor membr... | NM_016783.4 | 91.6% | .3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 363
- ORF length:
- 297
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tgccgaggag tggtggcgac tggcgccgac ccaagcgatc tggagagcgg 121 cgggctgctg catgagattt tcacgtcgcc gctcaacctg ctgctgcttg gcctctgcat 181 cttcctgctc tacaagatcg tgcgcgggga ccagccggcg gccagcggcg acagcgacga 241 cgacgagccg ccccctctgc cccgcctcaa gcggcgcgac ttcacccccg ccgagctgcg 301 gcgcttcgac ggcgtccagg acccgcgcat actcatggcc atcaacggca aggtgttcga 361 tgtgaccaaa ggccgcaaat tctacgggcc cgaggggccg tatggggtct ttgctggaag 421 agatgcatcc aggggccttg ccacattttg cctggataag gaagcactga aggatgagta 481 cgatgacctt tctgacctca ctgctgccca gcaggagact ctgagtgact gggagtctca 541 gttcactttc aagtatcatc acgtgggcaa actgctgaag gagggggagg agcccactgt 601 gtactcagat gaggaagaac caaaagatga gagtgcccgg aaaaatgatt acccaacttt 661 cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg attctacgta 721 gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct ggatta