Construct: ORF ccsbBroad304_14217
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012421.1_s304c1, BRDN0000400375
- Derived from:
- ccsbBroadEn_14217
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCDHA2 (56146)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14217
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56146 | PCDHA2 | protocadherin alpha 2 | NM_031496.2 | 100% | 100% | |
| 2 | human | 56146 | PCDHA2 | protocadherin alpha 2 | NM_031495.2 | 97% | 96.6% | (many diffs) |
| 3 | human | 56146 | PCDHA2 | protocadherin alpha 2 | NM_018905.3 | 84.7% | 84% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2490
- ORF length:
- 2424
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gtcttctatc agaaggggcc gaggggcctg gacacggctg ctctcgcttc 121 tgctcctcgc agcctgggag gtggggagcg gccagctccg ctactccgtc cccgaggagg 181 ccaaacacgg caccttcgtg ggccgcatcg cgcaggacct ggggctggag ctggaggagc 241 tggtgccgcg cctgttccgg gtggcgtcca aaagacacgg ggaccttctg gaggtaaatc 301 tgcagaatgg cattttgttt gtgaattctc ggatcgaccg ggaggagctg tgcgggcgga 361 gcgcggaatg tagcatccac gtggaggtga tcgtggacag gccgctgcag gttttccatg 421 tggaagtgga ggtgaaggac attaacgaca acccgccaat atttccaatg acagtaaaga 481 ctatccggtt tcccgaatca aggctgcttg attctcggtt tcctctagag ggagcatctg 541 atgcagatat aggagtaaat gctcttctct cctacaagct cagctccagt gagtttttct 601 tcctagatat acaggcaaat gatgaactaa gcgaatcttt gtctctcgtg ctggggaaat 661 cgctggacag agaggaaact gctgaggtta atttgttact ggtggctact gatgggggca 721 aacctgagct cacgggcacc gttcaaatac ttattaaggt attagatgta aatgacaatg 781 aaccaacttt tgcccaatca gtttacaaag taaaattgtt agagaatacg gcaaatggga 841 ccttagtggt taagttaaac gcttctgatg cagatgaagg accgaacagc gagattgtgt 901 attcactcgg tagtgatgtg tcctccacta tacagactaa gtttaccata gatcccatct 961 caggggaaat cagaactaag ggaaaattag attatgaaga agcaaagtcc tacgagattc 1021 aggtcactgc aactgacaaa ggaacccctt caatgtcagg acattgtaaa atttcattaa 1081 aacttgtgga catcaatgat aacacaccag aagtctcaat aacgtctctc tcacttccca 1141 tctcagagaa cgcttccctg ggcactgtca ttgctctcat cacggtgtcg gatcgcgact 1201 ctggtacgaa tggacatgtc acctgctccc tgacgcccca cgtccctttc aagctggtgt 1261 ccaccttcaa gaattactac tcgttggtgc tggacagcgc cctggaccgc gagagcgtgt 1321 cagcctatga gctggtggtg accgcacggg acgggggctc gccttcactg tgggccacca 1381 ccagcgtgtc catcgaggtg gccgacgtga acgacaacgc gccggcgttc gcacagcctg 1441 agtacacagt attcgtgaag gagaacaacc cgccgggctg ccacatcttc acggtgtcag 1501 cgtgggatgc ggacgcgcag gagaacgcgc tggtgtccta ctcgctggtg gagcggcggg 1561 tgggcgagcg cgcgttgtcg agctacgttt cggtgcacgc ggagagcggc aaggtgtacg 1621 cgctgcagcc gctggaccac gaggaagtgg agctgctgca gttccaggtg agcgcgcggg 1681 atgcgggcgt gccgcctctg ggcagcaacg tgacgctgca ggtgttcgtg ctggacgaga 1741 acgacaacgc gccggcactg ttggcgccta gggctggcac cgctgctggc gcagtgagtg 1801 agctggtgcc gtggtcggtg ggtgcagggc acgtggtggc gaaggtgcgc gcagtggacg 1861 ctgactcagg ctacaacgcg tggctttcgt acgagcttca gctgggtact ggcagcgctc 1921 gcatcccgtt ccgcgtgggg ctatacacgg gtgagatcag cacgacacgt gccctagacg 1981 aggctgactc ccctcgacac cgcctactcg tgctggtgaa ggaccacggc gaaccagcgt 2041 tgacagccac ggccaccgtg ttagtgtcgt tggtggaaag tggccaggca cccaaggcct 2101 cgtcgcgggc gtgggtgggc gccgcgggct cagaggctac gctggtggat gtcaacgtgt 2161 acctgatcat cgccatctgc gcggtatcca gcctgttggt gctcacggtg ctgctgtaca 2221 ctgcgctgcg gtgctcggtg ccacccaccg agggtgcgcg cgcgccagga aagcccacgc 2281 tggtgtgctc cagcgccgtg gggagctggt cttactcgca gcagaggcgg cagagggtgt 2341 gctctgggga ggaccccccc aagacggacc tcatggcctt cagccctagc ttatctcaag 2401 gtccagactc cgcagaagag aaacagctct cagaatcaga atacgtagga aagatatgga 2461 acttcaatct tcaaatccag cttgcctctt cccaactttc ttgtacaaag tggttggtaa 2521 gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta gcgctaaccg 2581 gtggcgcgtt aagtcgacaa tcaacctctg gatta