Construct: ORF ccsbBroad304_14284
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002744.1_s304c1, BRDN0000402269
- Derived from:
- ccsbBroadEn_14284
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ULBP2 (80328)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14284
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80328 | ULBP2 | UL16 binding protein 2 | NM_025217.4 | 99.7% | 99.1% | 369A>C;733delG |
| 2 | human | 154064 | RAET1L | retinoic acid early transcr... | NM_130900.3 | 95.9% | 93.4% | (many diffs) |
| 3 | human | 80328 | ULBP2 | UL16 binding protein 2 | XM_017011321.1 | 85.7% | 85.3% | 369A>C;631_632insCACCACTCGCCAT;633_634ins91 |
| 4 | human | 353091 | RAET1G | retinoic acid early transcr... | XM_011535800.3 | 78.1% | 71.2% | (many diffs) |
| 5 | human | 353091 | RAET1G | retinoic acid early transcr... | NM_001001788.4 | 69.4% | 63.3% | (many diffs) |
| 6 | human | 353091 | RAET1G | retinoic acid early transcr... | XM_017010831.2 | 68.6% | 62.6% | (many diffs) |
| 7 | human | 353091 | RAET1G | retinoic acid early transcr... | NR_130110.2 | 62% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 804
- ORF length:
- 738
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc agcagccgcc gctaccaaga tccttctgtg cctcccgctt ctgctcctgc 121 tgtccggctg gtcccgggct gggcgagccg accctcactc tctttgctat gacatcaccg 181 tcatccctaa gttcagacct ggaccacggt ggtgtgcggt tcaaggccag gtggatgaaa 241 agacttttct tcactatgac tgtggcaaca agacagtcac acctgtcagt cccctgggga 301 agaaactaaa tgtcacaacg gcctggaaag cacagaaccc agtactgaga gaggtggtgg 361 acatacttac agagcaactg cgtgacattc agctggagaa ttacacaccc aaggaacccc 421 tcaccctgca ggccaggatg tcttgtgagc agaaagctga aggacacagc agtggatctt 481 ggcagttcag tttcgatggg cagatcttcc tcctctttga ctcagagaag agaatgtgga 541 caacggttca tcctggagcc agaaagatga aagaaaagtg ggagaatgac aaggttgtgg 601 ccatgtcctt ccattacttc tcaatgggag actgtatagg atggcttgag gacttcttga 661 tgggcatgga cagcaccctg gagccaagtg caggagcacc actcgccatg tcctcaggca 721 caacccaact cagggccaca gccaccaccc tcatcctttg ctgcctcctc atcatcctcc 781 cctgcttcat cctccctgca tctgcccaac tttcttgtac aaagtggttg gtaagcctat 841 ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta accggtggcg 901 cgttaagtcg acaatcaacc tctggatta