Construct: ORF ccsbBroad304_14803
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018856.1_s304c1, BRDN0000459340
- Derived from:
- ccsbBroadEn_14803
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAPK8 (5599)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14803
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
No results found.
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 234
- ORF length:
- 165
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgaccta atagatgggt actgccggct ggtgaatgga acctcgcagt 121 catttatcat cagacctcag aaaggtgagg tgctgtttct gagcattccc aattgcgctt 181 ctaccaaata tttagctgtg cttgccccta acagcctgtt atcctctttt aggtaactgt 241 aaaaatgaag accaaatgaa taattcagct cagtataatc tgaggactta tttttctagg 301 gatggtgatc cgtttcacac cttccacaag ccaagagctg ggtttgaatt gccaactttc 361 ttgtacaaag ttggcattat aaganagctn ngtttgaatt gccaacttnc ttntacaann 421 tnnngcaacg aacaggtcac tatcnntnan aanaaaanca ttgcttatca atattnnnnn 481 nncngnnnan ntcnctatca gtatannnan nngtattaca nnntcatagc tgtttcctgg 541 cagctctggc ccgtgtctca aaatntntgc tatccagntg ntncaaatcg aggcgccgaa 601 caaaaaagct cctactattt tgttaaaaaa cccaagtcgt tctatagctg tggatgtgta 661 tctaccacct cttcattctc gagacggcag agctgcccgc aaagcacgtt ttgctctagc 721 actgatctct tcaaatcata caaaagctat tgacctctct tgttgtacta agttggtatt 781 atacacagag tgattatcat ttgttgcttc gtagtgaccg tagagaaaaa tacactatta 841 gtgtcccctg atgatcgccc gaccagagag tcatgaatct atggcagaac tcctcccaac 901 agtaaggact tacgttgaaa acagacctaa atatgctgga tatagctttG AGAAACTCTT 961 CCCTGATGTC CTTTTCCCAG CTGACTCAGA ACACAACAAA CTTAAAGCCA GTCAGGCAAG 1021 GGATTTGTTA TCCAAAATGC TGGTAATAGA TGCATCTAAA AGGATCTCTG TAGATGAAGC 1081 TCTCCAACAC CCGTACATCA ATGTCTGGTA TGATCCTTCT GAAGCAGAAG CTCCACCACC 1141 AAAGATCCCT GACAAGCAGT TAGATGAAAG GGAACACACA ATAGAAGAGT GGAAAGAATT 1201 GATATATAAG GAAGTTATGG ACTTGGAGGA GAGAACCAAG AATGGAGTTA TACGGGGGCA 1261 GCCCTCTCCT TTAGGTGCAG CAGTGATCAA TGGCTCTCAG CATCCATCAT CATCGTCGTC 1321 TGTCAATGAT GTGTCTTCAA TGTCAACAGA TCCGACTTTG GCCTCTGATA CAGACAGCAG 1381 TCTAGAAGCA GCAGCTGGGC CTCTGGGCTG CTGTAGATTG CCAACTTTCT TGTACaaagt 1441 ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt aatgagctag 1501 cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta