Construct: ORF ccsbBroad304_14971
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011122.1_s304c1, BRDN0000459687
- Derived from:
- ccsbBroadEn_14971
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PMVK (10654)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14971
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10654 | PMVK | phosphomevalonate kinase | NM_006556.4 | 100% | 100% | |
| 2 | human | 10654 | PMVK | phosphomevalonate kinase | NM_001323011.3 | 84.4% | 86.9% | (many diffs) |
| 3 | human | 10654 | PMVK | phosphomevalonate kinase | NM_001323012.3 | 60.9% | 60.9% | 0_1ins225 |
| 4 | human | 10654 | PMVK | phosphomevalonate kinase | NM_001348696.2 | 60.9% | 53.6% | 0_1ins106;50_51ins119 |
| 5 | mouse | 68603 | Pmvk | phosphomevalonate kinase | NM_026784.3 | 86.6% | 83.3% | (many diffs) |
| 6 | mouse | 68603 | Pmvk | phosphomevalonate kinase | NM_001310640.1 | 52.4% | 52% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 642
- ORF length:
- 576
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cccgctggga ggcgccccgc ggctggtact gctgttcagc ggcaagagga 121 aatccgggaa ggacttcgtg accgaggcgc tgcagagcag acttggagct gatgtctgtg 181 ctgtcctccg gctctctggt ccactcaagg aacagtatgc tcaggagcat ggcttgaact 241 tccagagact cctggacacc agcacctaca aggaggcctt tcggaaggac atgatccgct 301 ggggagagga gaaacgccag gctgacccag gcttcttttg caggaagatt gtggagggca 361 tctcccagcc catctggctg gtgagtgaca cacggagagt gtctgacatc cagtggtttc 421 gggaggccta tggggccgtg acgcagacgg tccgcgttgt agcgttggag cagagccgac 481 agcagcgggg ctgggtgttc acgccagggg tggacgatgc tgagtcagaa tgtggcctgg 541 acaacttcgg ggactttgac tgggtcatcg agaaccatgg agttgaacag cgcctggagg 601 agcagttgga gaacctgata gaatttatcc gctccagact ttacccaact ttcttgtaca 661 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 721 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta