Construct: ORF ccsbBroad304_15168

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000063.1_s304c1, BRDN0000401242
Derived from:
ccsbBroadEn_15168
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EFHD1 (80303)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15168
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 80303 EFHD1 EF-hand domain family membe... NM_025202.4 100% 100%
2 human 80303 EFHD1 EF-hand domain family membe... NM_001243252.1 59.4% 58.3% (many diffs)
3 human 80303 EFHD1 EF-hand domain family membe... NM_001308395.2 53.1% 53.1% 0_1ins336
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
783
ORF length:
717
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc cagtgaggag ctggcgtgca agctggagcg ccggctgcgg cgcgaggagg
121ccgaggagag tggcccccag ctggctcccc tcggcgcccc agccccggag cccaagcccg
181agcccgagcc tcccgcccgt gcgcccacgg ccagcgccga cgcggagctg agcgcccagc
241tgagccggcg gctggacatc aacgagggcg ctgcgcggcc ccggcgctgc agggtcttca
301acccctacac ggagttcccg gagttcagcc gccgcctcat caaggacctg gagagcatgt
361tcaaactgta tgacgctggg cgggatggct tcatcgacct gatggagctg aagctgatga
421tggagaagct gggggccccc cagacccacc tgggcctgaa gagcatgatc aaggaggtgg
481atgaggactt cgatggcaag ctcagcttcc gggagttcct gctcattttc cacaaggccg
541cggcagggga gctgcaggag gacagtgggc tgatggcgct ggcaaagctt tctgagatcg
601atgtggccct ggagggtgtc aaaggtgcca agaacttctt tgaagccaag gtccaagcct
661tgtcatcggc cagtaagttt gaagcagagt tgaaagctga gcaagatgag cggaagcggg
721aggaggagga gaggcggctc cgccaggcag ccttccagaa actcaaggcc aacttcaata
781catacccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
841tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
901tctggatta

Download FASTA (ORF) (Full)