Construct: ORF ccsbBroad304_15277
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015846.2_s304c1, BRDN0000459514
- Derived from:
- ccsbBroadEn_15277
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NIM1K (167359)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15277
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 167359 | NIM1K | NIM1 serine/threonine prote... | NM_153361.4 | 99.9% | 100% | 45C>T |
| 2 | human | 167359 | NIM1K | NIM1 serine/threonine prote... | NR_072980.1 | 44.3% | 1_1499del;1544C>T;2808_2944del | |
| 3 | mouse | 245269 | Nim1k | NIM1 serine/threonine prote... | NM_175538.3 | 87.3% | 91.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1374
- ORF length:
- 1308
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac tgcagtgtat atgaatggag gtggcctggt gaacccccat tatgcccggt 121 gggatcggcg cgacagtgta gaaagtggct gtcagaccga gagtagcaag gagggtgagg 181 agggacagcc ccgccagctg acgcccttcg agaaactgac acaggacatg tcccaggatg 241 agaaggtggt gagggagatc acgctgggga aacggatagg cttctaccga attcgagggg 301 aaatcggaag tggaaacttc tcccaagtga agcttgggat tcactcccta accaaagaaa 361 aggtggccat taagatcctg gacaagacca agttagacca gaaaacccag aggctactat 421 cccgagaaat ctccagcatg gaaaagctgc accatcccaa catcatccgc ctttacgaag 481 tggtggagac cctatccaag ctgcacttgg tgatggagta tgcagggggt ggggagctct 541 tcggaaaaat tagcactgag gggaagctct ctgaaccaga aagcaagctc atcttctccc 601 agattgtgtc tgccgtgaag cacatgcatg aaaaccaaat tattcataga gatctgaaag 661 cagaaaatgt attctatacc agtaatactt gtgtgaaggt gggcgatttt ggattcagca 721 cagtaagcaa aaaaggtgaa atgctgaaca ctttctgtgg gtctcctccc tacgctgcgc 781 ctgaactctt ccgggacgag cactacatcg gcatttacgt ggatatctgg gccttggggg 841 tgcttttgta cttcatggtg actggcacca tgccatttcg ggcagaaacc gtggccaaac 901 taaaaaagag catcctcgag ggcacataca gtgtaccgcc gcacgtgtca gagccctgcc 961 accgactcat ccgaggagtc cttcagcaga tccccacgga gaggtacgga atcgactgca 1021 tcatgaatga tgaatggatg caaggggtgc cataccctac acctttggaa cctttccaac 1081 tggatcccaa acatttgtcg gaaaccagca ctctcaagga agaagaaaat gaggtcaaaa 1141 gcactttaga acatttgggc attacagaag agcatattcg aaataaccaa gggagagatg 1201 ctcgcagctc aatcacaggg gtctatagaa ttattttaca tagagtccaa aggaagaagg 1261 ctttggaaag tgtcccagtc atgatgctac cagaccctaa agaaagagac ctcaaaaaag 1321 ggtcccgtgt ctacagaggg ataagacaca catccaaatt ttgctcgatt ttatacccaa 1381 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1441 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta