Construct: ORF ccsbBroad304_15781
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009902.1_s304c1, BRDN0000402359
- Derived from:
- ccsbBroadEn_15781
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SERBP1 (26135)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15781
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26135 | SERBP1 | SERPINE1 mRNA binding prote... | NM_015640.4 | 100% | 100% | |
| 2 | human | 26135 | SERBP1 | SERPINE1 mRNA binding prote... | NM_001018069.2 | 98.4% | 98.4% | 606_623del |
| 3 | human | 26135 | SERBP1 | SERPINE1 mRNA binding prote... | NM_001018068.2 | 96.2% | 96.2% | 678_722del |
| 4 | human | 26135 | SERBP1 | SERPINE1 mRNA binding prote... | NM_001018067.2 | 94.8% | 94.8% | 606_623del;696_740del |
| 5 | mouse | 66870 | Serbp1 | serpine1 mRNA binding prote... | NM_001113566.1 | 93% | 98.4% | (many diffs) |
| 6 | mouse | 66870 | Serbp1 | serpine1 mRNA binding prote... | NM_001113565.1 | 91.6% | 96.9% | (many diffs) |
| 7 | mouse | 66870 | Serbp1 | serpine1 mRNA binding prote... | NM_001113564.1 | 89.5% | 94.7% | (many diffs) |
| 8 | mouse | 66870 | Serbp1 | serpine1 mRNA binding prote... | NM_025814.2 | 88.2% | 93.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1227
- ORF length:
- 1161
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc tgggcactta caggaaggct tcggctgcgt ggtcaccaac cgattcgacc 121 agttatttga cgacgaatcg gaccccttcg aggtgctgaa ggcagcagag aacaagaaaa 181 aagaagccgg cgggggcggc gttgggggcc ctggggccaa gagcgcagct caggccgcgg 241 cccagaccaa ctccaacgcg gcaggcaaac agctgcgcaa ggagtcccag aaagaccgca 301 agaacccgct gccccccagc gttggcgtgg ttgacaagaa agaggagacg cagccgcccg 361 tggcgcttaa gaaagaagga ataagacgag ttggaagaag acctgatcaa caacttcagg 421 gtgaagggaa aataattgat agaagaccag aaaggcgacc acctcgtgaa cgaagattcg 481 aaaagccact tgaagaaaag ggtgaaggag gcgaattttc agttgataga ccgattattg 541 accgacctat tcgaggtcgt ggtggtcttg gaagaggtcg agggggccgt ggacgtggaa 601 tgggccgagg agatggattt gattctcgtg gcaaacgtga atttgatagg catagtggaa 661 gtgatagatc tggcctgaag cacgaggaca aacgtggagg tagcggatct cacaactggg 721 gaactgtcaa agacgaatta actgacttgg atcaatcaaa tgtgactgag gaaacacctg 781 aaggtgaaga acatcatcca gtggcagaca ctgaaaataa ggagaatgaa gttgaagagg 841 taaaagagga gggtccaaaa gagatgactt tggatgagtg gaaggctatt caaaataagg 901 accgggcaaa agtagaattt aatatccgaa aaccaaatga aggtgctgat gggcagtgga 961 agaagggatt tgttcttcat aaatcaaaga gtgaagaggc tcatgctgaa gattcggtta 1021 tggaccatca tttccggaag ccagcaaatg atataacgtc tcagctggag atcaattttg 1081 gagaccttgg ccgcccagga cgtggcggca ggggaggacg aggtggacgt gggcgtggtg 1141 ggcgcccaaa ccgtggcagc aggaccgaca agtcaagtgc ttctgctcct gatgtggatg 1201 acccagaggc attcccagct ctggcttnnc caactttctt gtacaaagtg gttggtaagc 1261 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 1321 ggcgcgttaa gtcgacaatc aacctctgga tta